p53 protein accumulation in European hepatocellular carcinoma is not always dependent on p53 gene mutation

Jean-Christophe Bourdon, Antonia D'Errico, Patrizia Paterlini, Walter Grigioni, Evelyne May, Brigitte Debuire

    Research output: Contribution to journalArticle

    54 Citations (Scopus)

    Abstract

    Background/Aims: Immunohistochemical reactivity for p53 protein is; common in various human malignancies and often related to p53 gene mutation. However, in some tumor types, accumulation of wild-type p53 has been shown. previously, we analyzed 96 European hepatocellular carcinomas using immunohistochemistry and found that 31% of these tumors overexpressed p53 in the cell nucleus. the aim of the present study was to establish whether p53 positivity correlates with the presence of structural p53 gene abnormalities in European hepatocellular carcinoma. Methods: DNA from 20 tumors, 10 with strong immunostaining and 10 with undetectable staining for p53, was extracted from frozen sections, and the entire coding portion of the p53 gene was sequenced. Results: Five of the 10 tumors containing high levels of p53 protein showed missense point mutations. The remaining 5 tumors with high p53 levels showed the wild-type coding sequence. One of the 10 tumors containing undetectable levels of p53 protein had a 1-base pair deletion in the splice acceptor site of intron 4. Conclusions: The results strongly suggest that, in European hepatocellular carcinomas, stabilization of the p53 protein depends on factors other than p53 gene mutation, such as binding to other molecules of cellular or viral origin.

    Original languageEnglish
    Pages (from-to)1176-1182
    Number of pages7
    JournalGastroenterology
    Volume108
    Issue number4
    DOIs
    Publication statusPublished - Apr 1995

    Cite this

    Bourdon, Jean-Christophe ; D'Errico, Antonia ; Paterlini, Patrizia ; Grigioni, Walter ; May, Evelyne ; Debuire, Brigitte . / p53 protein accumulation in European hepatocellular carcinoma is not always dependent on p53 gene mutation. In: Gastroenterology. 1995 ; Vol. 108, No. 4. pp. 1176-1182.
    @article{d95e34691f0e4894a1681f2a5bdd6af8,
    title = "p53 protein accumulation in European hepatocellular carcinoma is not always dependent on p53 gene mutation",
    abstract = "Background/Aims: Immunohistochemical reactivity for p53 protein is; common in various human malignancies and often related to p53 gene mutation. However, in some tumor types, accumulation of wild-type p53 has been shown. previously, we analyzed 96 European hepatocellular carcinomas using immunohistochemistry and found that 31{\%} of these tumors overexpressed p53 in the cell nucleus. the aim of the present study was to establish whether p53 positivity correlates with the presence of structural p53 gene abnormalities in European hepatocellular carcinoma. Methods: DNA from 20 tumors, 10 with strong immunostaining and 10 with undetectable staining for p53, was extracted from frozen sections, and the entire coding portion of the p53 gene was sequenced. Results: Five of the 10 tumors containing high levels of p53 protein showed missense point mutations. The remaining 5 tumors with high p53 levels showed the wild-type coding sequence. One of the 10 tumors containing undetectable levels of p53 protein had a 1-base pair deletion in the splice acceptor site of intron 4. Conclusions: The results strongly suggest that, in European hepatocellular carcinomas, stabilization of the p53 protein depends on factors other than p53 gene mutation, such as binding to other molecules of cellular or viral origin.",
    author = "Jean-Christophe Bourdon and Antonia D'Errico and Patrizia Paterlini and Walter Grigioni and Evelyne May and Brigitte Debuire",
    year = "1995",
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    doi = "10.1016/0016-5085(95)90217-1",
    language = "English",
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    p53 protein accumulation in European hepatocellular carcinoma is not always dependent on p53 gene mutation. / Bourdon, Jean-Christophe; D'Errico, Antonia ; Paterlini, Patrizia ; Grigioni, Walter ; May, Evelyne ; Debuire, Brigitte .

    In: Gastroenterology, Vol. 108, No. 4, 04.1995, p. 1176-1182.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - p53 protein accumulation in European hepatocellular carcinoma is not always dependent on p53 gene mutation

    AU - Bourdon, Jean-Christophe

    AU - D'Errico, Antonia

    AU - Paterlini, Patrizia

    AU - Grigioni, Walter

    AU - May, Evelyne

    AU - Debuire, Brigitte

    PY - 1995/4

    Y1 - 1995/4

    N2 - Background/Aims: Immunohistochemical reactivity for p53 protein is; common in various human malignancies and often related to p53 gene mutation. However, in some tumor types, accumulation of wild-type p53 has been shown. previously, we analyzed 96 European hepatocellular carcinomas using immunohistochemistry and found that 31% of these tumors overexpressed p53 in the cell nucleus. the aim of the present study was to establish whether p53 positivity correlates with the presence of structural p53 gene abnormalities in European hepatocellular carcinoma. Methods: DNA from 20 tumors, 10 with strong immunostaining and 10 with undetectable staining for p53, was extracted from frozen sections, and the entire coding portion of the p53 gene was sequenced. Results: Five of the 10 tumors containing high levels of p53 protein showed missense point mutations. The remaining 5 tumors with high p53 levels showed the wild-type coding sequence. One of the 10 tumors containing undetectable levels of p53 protein had a 1-base pair deletion in the splice acceptor site of intron 4. Conclusions: The results strongly suggest that, in European hepatocellular carcinomas, stabilization of the p53 protein depends on factors other than p53 gene mutation, such as binding to other molecules of cellular or viral origin.

    AB - Background/Aims: Immunohistochemical reactivity for p53 protein is; common in various human malignancies and often related to p53 gene mutation. However, in some tumor types, accumulation of wild-type p53 has been shown. previously, we analyzed 96 European hepatocellular carcinomas using immunohistochemistry and found that 31% of these tumors overexpressed p53 in the cell nucleus. the aim of the present study was to establish whether p53 positivity correlates with the presence of structural p53 gene abnormalities in European hepatocellular carcinoma. Methods: DNA from 20 tumors, 10 with strong immunostaining and 10 with undetectable staining for p53, was extracted from frozen sections, and the entire coding portion of the p53 gene was sequenced. Results: Five of the 10 tumors containing high levels of p53 protein showed missense point mutations. The remaining 5 tumors with high p53 levels showed the wild-type coding sequence. One of the 10 tumors containing undetectable levels of p53 protein had a 1-base pair deletion in the splice acceptor site of intron 4. Conclusions: The results strongly suggest that, in European hepatocellular carcinomas, stabilization of the p53 protein depends on factors other than p53 gene mutation, such as binding to other molecules of cellular or viral origin.

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