Abstract
1. The partial purification of two lithocholic acid-binding proteins from liver 100 000g supernatants is described. 2. Gel-filtration, (NH4)2SO4 fractionation, Ca3(PO4)2 fractionation and ion-exchange chromatography were used. 3. Both proteins exhibited glutathione S-transferase activity; one may be the non-specific anion-binding protein ligandin. 4. Glutathione S-transferase activity of one of the binding proteins was inhibited by lithocholic acid.
Original language | English |
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Pages (from-to) | 425-9 |
Number of pages | 5 |
Journal | Biochemical Journal |
Volume | 165 |
Issue number | 3 |
Publication status | Published - 1 Sept 1977 |
Keywords
- Animals
- Bile Acids and Salts/pharmacology
- Carrier Proteins/isolation & purification
- Chromatography, Ion Exchange
- Glutathione Transferase/antagonists & inhibitors
- Lithocholic Acid
- Liver/analysis
- Male
- Rats