Partial purification of two lithocholic acid-binding proteins from rat liver 100 000g supernatants

R C Strange, R Cramb, J D Hayes, I W Percy-Robb

    Research output: Contribution to journalArticle

    22 Citations (Scopus)

    Abstract

    1. The partial purification of two lithocholic acid-binding proteins from liver 100 000g supernatants is described. 2. Gel-filtration, (NH4)2SO4 fractionation, Ca3(PO4)2 fractionation and ion-exchange chromatography were used. 3. Both proteins exhibited glutathione S-transferase activity; one may be the non-specific anion-binding protein ligandin. 4. Glutathione S-transferase activity of one of the binding proteins was inhibited by lithocholic acid.

    Original languageEnglish
    Pages (from-to)425-9
    Number of pages5
    JournalBiochemical Journal
    Volume165
    Issue number3
    Publication statusPublished - 1 Sep 1977

    Fingerprint

    Lithocholic Acid
    Glutathione Transferase
    Liver
    Purification
    Rats
    Carrier Proteins
    Fractionation
    Ion Exchange Chromatography
    Chromatography
    Gel Chromatography
    Anions
    Ion exchange
    Gels
    Proteins

    Keywords

    • Animals
    • Bile Acids and Salts/pharmacology
    • Carrier Proteins/isolation & purification
    • Chromatography, Ion Exchange
    • Glutathione Transferase/antagonists & inhibitors
    • Lithocholic Acid
    • Liver/analysis
    • Male
    • Rats

    Cite this

    Strange, R C ; Cramb, R ; Hayes, J D ; Percy-Robb, I W. / Partial purification of two lithocholic acid-binding proteins from rat liver 100 000g supernatants. In: Biochemical Journal. 1977 ; Vol. 165, No. 3. pp. 425-9.
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    abstract = "1. The partial purification of two lithocholic acid-binding proteins from liver 100 000g supernatants is described. 2. Gel-filtration, (NH4)2SO4 fractionation, Ca3(PO4)2 fractionation and ion-exchange chromatography were used. 3. Both proteins exhibited glutathione S-transferase activity; one may be the non-specific anion-binding protein ligandin. 4. Glutathione S-transferase activity of one of the binding proteins was inhibited by lithocholic acid.",
    keywords = "Animals, Bile Acids and Salts/pharmacology, Carrier Proteins/isolation & purification, Chromatography, Ion Exchange, Glutathione Transferase/antagonists & inhibitors, Lithocholic Acid, Liver/analysis, Male, Rats",
    author = "Strange, {R C} and R Cramb and Hayes, {J D} and Percy-Robb, {I W}",
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    Partial purification of two lithocholic acid-binding proteins from rat liver 100 000g supernatants. / Strange, R C; Cramb, R; Hayes, J D; Percy-Robb, I W.

    In: Biochemical Journal, Vol. 165, No. 3, 01.09.1977, p. 425-9.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Partial purification of two lithocholic acid-binding proteins from rat liver 100 000g supernatants

    AU - Strange, R C

    AU - Cramb, R

    AU - Hayes, J D

    AU - Percy-Robb, I W

    PY - 1977/9/1

    Y1 - 1977/9/1

    N2 - 1. The partial purification of two lithocholic acid-binding proteins from liver 100 000g supernatants is described. 2. Gel-filtration, (NH4)2SO4 fractionation, Ca3(PO4)2 fractionation and ion-exchange chromatography were used. 3. Both proteins exhibited glutathione S-transferase activity; one may be the non-specific anion-binding protein ligandin. 4. Glutathione S-transferase activity of one of the binding proteins was inhibited by lithocholic acid.

    AB - 1. The partial purification of two lithocholic acid-binding proteins from liver 100 000g supernatants is described. 2. Gel-filtration, (NH4)2SO4 fractionation, Ca3(PO4)2 fractionation and ion-exchange chromatography were used. 3. Both proteins exhibited glutathione S-transferase activity; one may be the non-specific anion-binding protein ligandin. 4. Glutathione S-transferase activity of one of the binding proteins was inhibited by lithocholic acid.

    KW - Animals

    KW - Bile Acids and Salts/pharmacology

    KW - Carrier Proteins/isolation & purification

    KW - Chromatography, Ion Exchange

    KW - Glutathione Transferase/antagonists & inhibitors

    KW - Lithocholic Acid

    KW - Liver/analysis

    KW - Male

    KW - Rats

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