Partial purification of two lithocholic acid-binding proteins from rat liver 100 000g supernatants

R C Strange, R Cramb, J D Hayes, I W Percy-Robb

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    22 Citations (Scopus)

    Abstract

    1. The partial purification of two lithocholic acid-binding proteins from liver 100 000g supernatants is described. 2. Gel-filtration, (NH4)2SO4 fractionation, Ca3(PO4)2 fractionation and ion-exchange chromatography were used. 3. Both proteins exhibited glutathione S-transferase activity; one may be the non-specific anion-binding protein ligandin. 4. Glutathione S-transferase activity of one of the binding proteins was inhibited by lithocholic acid.

    Original languageEnglish
    Pages (from-to)425-9
    Number of pages5
    JournalBiochemical Journal
    Volume165
    Issue number3
    Publication statusPublished - 1 Sep 1977

    Keywords

    • Animals
    • Bile Acids and Salts/pharmacology
    • Carrier Proteins/isolation & purification
    • Chromatography, Ion Exchange
    • Glutathione Transferase/antagonists & inhibitors
    • Lithocholic Acid
    • Liver/analysis
    • Male
    • Rats

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