Partial purification of two lithocholic acid-binding proteins from rat liver 100 000g supernatants

TY - JOUR

T1 - Partial purification of two lithocholic acid-binding proteins from rat liver 100 000g supernatants

AU - Strange, R C

AU - Cramb, R

AU - Hayes, J D

AU - Percy-Robb, I W

PY - 1977/9/1

Y1 - 1977/9/1

N2 - 1. The partial purification of two lithocholic acid-binding proteins from liver 100 000g supernatants is described. 2. Gel-filtration, (NH4)2SO4 fractionation, Ca3(PO4)2 fractionation and ion-exchange chromatography were used. 3. Both proteins exhibited glutathione S-transferase activity; one may be the non-specific anion-binding protein ligandin. 4. Glutathione S-transferase activity of one of the binding proteins was inhibited by lithocholic acid.

AB - 1. The partial purification of two lithocholic acid-binding proteins from liver 100 000g supernatants is described. 2. Gel-filtration, (NH4)2SO4 fractionation, Ca3(PO4)2 fractionation and ion-exchange chromatography were used. 3. Both proteins exhibited glutathione S-transferase activity; one may be the non-specific anion-binding protein ligandin. 4. Glutathione S-transferase activity of one of the binding proteins was inhibited by lithocholic acid.

KW - Animals

KW - Bile Acids and Salts/pharmacology

KW - Carrier Proteins/isolation & purification

KW - Chromatography, Ion Exchange

KW - Glutathione Transferase/antagonists & inhibitors

KW - Lithocholic Acid

KW - Liver/analysis

KW - Male

KW - Rats

UR - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1164923/

M3 - Article

C2 - 921757

SN - 0264-6021

VL - 165

SP - 425

EP - 429

JO - Biochemical Journal

JF - Biochemical Journal

IS - 3

ER -