Abstract
The BMP and Wnt signalling pathways determine axis specification during embryonic development. Our previous work has shown that PAWS1 (also known as FAM83G) interacts with SMAD1 and modulates BMP signalling. Here, surprisingly, we show that overexpression of PAWS1 in Xenopus embryos activates Wnt signalling and causes complete axis duplication. Consistent with these observations in Xenopus, Wnt signalling is diminished in U2OS osteosarcoma cells lacking PAWS1, while BMP signalling is unaffected. We show that PAWS1 interacts and co-localises with the α isoform of casein kinase 1 (CK1), and that PAWS1 mutations incapable of binding CK1 fail both to activate Wnt signalling and to elicit axis duplication in Xenopus embryos.
Original language | English |
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Article number | e44807 |
Pages (from-to) | 1-18 |
Number of pages | 18 |
Journal | EMBO Reports |
Volume | 19 |
Issue number | 4 |
Early online date | 7 Mar 2018 |
DOIs | |
Publication status | Published - 1 Apr 2018 |
Keywords
- BMP
- CK1
- FAM83G
- PAWS1
- Wnt
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Genetics
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Dive into the research topics of 'PAWS1 controls Wnt signalling through association with casein kinase 1α'. Together they form a unique fingerprint.Student theses
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Elucidating the biological function of the uncharacterised protein FAM83G/PAWS1: Role in Wnt signalling through its interaction with Caseine Kinase 1α
Author: Bozatzi, P., 2018Supervisor: Sapkota, G. (Supervisor)
Student thesis: Doctoral Thesis › Doctor of Philosophy
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Identification of novel protein kinase regulators of TGFβ signalling
Author: Hutchinson, L., 2019Supervisor: Sapkota, G. (Supervisor)
Student thesis: Doctoral Thesis › Doctor of Philosophy
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