PAWS1 controls Wnt signalling through association with casein kinase 1α

Polyxeni Bozatzi, Kevin S. Dingwell, Kevin Z. L. Wu, Fay Cooper, Timothy D. Cummins, Luke D. Hutchinson, Janis Vogt, Nicola T. Wood, Thomas J. Macartney, Joby Varghese, Robert Gourlay, David G. Campbell, James C. Smith (Lead / Corresponding author), Gopal P. Sapkota (Lead / Corresponding author)

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16 Citations (Scopus)
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Abstract

The BMP and Wnt signalling pathways determine axis specification during embryonic development. Our previous work has shown that PAWS1 (also known as FAM83G) interacts with SMAD1 and modulates BMP signalling. Here, surprisingly, we show that overexpression of PAWS1 in Xenopus embryos activates Wnt signalling and causes complete axis duplication. Consistent with these observations in Xenopus, Wnt signalling is diminished in U2OS osteosarcoma cells lacking PAWS1, while BMP signalling is unaffected. We show that PAWS1 interacts and co-localises with the α isoform of casein kinase 1 (CK1), and that PAWS1 mutations incapable of binding CK1 fail both to activate Wnt signalling and to elicit axis duplication in Xenopus embryos.

Original languageEnglish
Article numbere44807
Pages (from-to)1-18
Number of pages18
JournalEMBO Reports
Volume19
Issue number4
Early online date7 Mar 2018
DOIs
Publication statusPublished - 1 Apr 2018

Keywords

  • BMP
  • CK1
  • FAM83G
  • PAWS1
  • Wnt

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