PDGF signalling controls the migration of mesoderm cells during chick gastrulation by regulating N-cadherin expression

Xuesong Yang, Holly Chrisman, Cornelis J. Weijer

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    73 Citations (Scopus)

    Abstract

    In the early chick embryo, Pdgfa is expressed in the epiblast, outlining the migration route that mesoderm cells expressing the receptor, Pdgfr alpha, follow to form somites. Both expression of a dominant-negative PDGFR alpha and depletion of endogenous PDGFRa ligands through injection of PDGFR alpha-Fc fragments, inhibit the migration of mesoderm cells after their ingression through the primitive streak. siRNA-mediated downregulation of Pdgfa expression in the epiblast on one side of the streak strongly blocks the migration of mesoderm cells into that side. Beads soaked in PDGFA elicit a directional attractive movement response in mesoderm cells, showing that PDGFA can provide directional information. Surprisingly, however, PDGF signalling is also required for directional movement towards other attractants, such as FGF4. PDGF signalling controls N-cadherin expression on mesoderm cells, which is required for efficient migration. PDGF signalling activates the PI3 kinase signalling pathway in vivo and activation of this pathway is required for proper N-cadherin expression.

    Original languageEnglish
    Pages (from-to)3521-3530
    Number of pages10
    JournalDevelopment
    Volume135
    Issue number21
    DOIs
    Publication statusPublished - 1 Nov 2008

    Keywords

    • Gastrulation
    • Cell movement
    • N-cadherin
    • PDGF signalling
    • PI3 kinase signalling
    • Adhesion molecules
    • Movement patterns
    • Primitive streak
    • Growth factor
    • Cleavage
    • Receptor
    • Embryo
    • ADAM10
    • Roles
    • 3-kinase

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