Background: The medical radiation science (MEDRADSC) collaborative program at Mohawk-McMaster (MoMac) Institute for Applied Health Sciences in Hamilton, Ontario, Canada, has used 360° feedback about the program since its inception in 2004, which is still ongoing. Aim: To measure students' perception of the educational environment in MEDRADSC using the Dundee Ready Education Environment Measure (DREEM), and investigate perception of the educational environment overall, by specialization, and by gender. Method: The DREEM instrument was distributed electronically in June 2009 to 105 third-year students from three medical radiation specializations: ultrasound, therapy, and radiography. Results: With an 83% response rate, students rated their educational climate overall as positive. No significant difference was found between gender or specialization at MoMac. Conclusion: The DREEM inventory can be used effectively within the medical radiation sciences to measure students' perceptions of educational environment and verify very specific issues relating to each of the five subscales.