Abstract
An extensive body of evidence supports the conclusion that by catalyzing obligatory two-electron reductions of quinones to hydroquinones, NAD(P)H:quinone reductase (QR1) protects cells against the deleterious effects of redox cycling of quinones, their ability to deplete glutathione, and to produce neoplasia. The effects of elevation of QR1 levels by various enzyme inducers, inhibition of the enzyme by dicumarol, and genetic deletion of the enzyme (knockout mouse) are all consistent with the proposed protective functions. Measurement of QR1 activity in murine hepatoma cells grown in 96-well microtiter plates has provided a rapid and quantitative method for detecting inducer activity and determining inducer potency. This constitutes a strategy for the identification of potential chemoprotectors against cancer. Epidemiological studies show that humans who are genetically deficient in QR1 are more susceptible to the hematological toxicity and carcinogenicity of benzene exposure, and may be more susceptible to the development of a number of malignant tumors. (C) 2000 Elsevier Science Inc.
| Original language | English |
|---|---|
| Pages (from-to) | 231-240 |
| Number of pages | 10 |
| Journal | Free Radical Biology and Medicine |
| Volume | 29 |
| Issue number | 3-4 |
| DOIs | |
| Publication status | Published - 1 Aug 2000 |
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Keywords
- Antioxidant response element (ARE)
- Benzene toxicity
- Chemoprotection
- Free radicals
- Hydroquinones
- Redox cycling
- Sulforaphane
Cite this
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Persuasive evidence that quinone reductase type 1 (DT diaphorase) protects cells against the toxicity of electrophiles and reactive forms of oxygen. / Dinkova-Kostova, Albena T.; Talalay, Paul.
In: Free Radical Biology and Medicine, Vol. 29, No. 3-4, 01.08.2000, p. 231-240.Research output: Contribution to journal › Review article
TY - JOUR
T1 - Persuasive evidence that quinone reductase type 1 (DT diaphorase) protects cells against the toxicity of electrophiles and reactive forms of oxygen
AU - Dinkova-Kostova, Albena T.
AU - Talalay, Paul
PY - 2000/8/1
Y1 - 2000/8/1
N2 - An extensive body of evidence supports the conclusion that by catalyzing obligatory two-electron reductions of quinones to hydroquinones, NAD(P)H:quinone reductase (QR1) protects cells against the deleterious effects of redox cycling of quinones, their ability to deplete glutathione, and to produce neoplasia. The effects of elevation of QR1 levels by various enzyme inducers, inhibition of the enzyme by dicumarol, and genetic deletion of the enzyme (knockout mouse) are all consistent with the proposed protective functions. Measurement of QR1 activity in murine hepatoma cells grown in 96-well microtiter plates has provided a rapid and quantitative method for detecting inducer activity and determining inducer potency. This constitutes a strategy for the identification of potential chemoprotectors against cancer. Epidemiological studies show that humans who are genetically deficient in QR1 are more susceptible to the hematological toxicity and carcinogenicity of benzene exposure, and may be more susceptible to the development of a number of malignant tumors. (C) 2000 Elsevier Science Inc.
AB - An extensive body of evidence supports the conclusion that by catalyzing obligatory two-electron reductions of quinones to hydroquinones, NAD(P)H:quinone reductase (QR1) protects cells against the deleterious effects of redox cycling of quinones, their ability to deplete glutathione, and to produce neoplasia. The effects of elevation of QR1 levels by various enzyme inducers, inhibition of the enzyme by dicumarol, and genetic deletion of the enzyme (knockout mouse) are all consistent with the proposed protective functions. Measurement of QR1 activity in murine hepatoma cells grown in 96-well microtiter plates has provided a rapid and quantitative method for detecting inducer activity and determining inducer potency. This constitutes a strategy for the identification of potential chemoprotectors against cancer. Epidemiological studies show that humans who are genetically deficient in QR1 are more susceptible to the hematological toxicity and carcinogenicity of benzene exposure, and may be more susceptible to the development of a number of malignant tumors. (C) 2000 Elsevier Science Inc.
KW - Antioxidant response element (ARE)
KW - Benzene toxicity
KW - Chemoprotection
KW - Free radicals
KW - Hydroquinones
KW - Redox cycling
KW - Sulforaphane
UR - http://www.scopus.com/inward/record.url?scp=0033805326&partnerID=8YFLogxK
U2 - 10.1016/S0891-5849(00)00300-2
DO - 10.1016/S0891-5849(00)00300-2
M3 - Review article
C2 - 11035251
AN - SCOPUS:0033805326
VL - 29
SP - 231
EP - 240
JO - Free Radical Biology and Medicine
JF - Free Radical Biology and Medicine
SN - 0891-5849
IS - 3-4
ER -