Persuasive evidence that quinone reductase type 1 (DT diaphorase) protects cells against the toxicity of electrophiles and reactive forms of oxygen

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Abstract

An extensive body of evidence supports the conclusion that by catalyzing obligatory two-electron reductions of quinones to hydroquinones, NAD(P)H:quinone reductase (QR1) protects cells against the deleterious effects of redox cycling of quinones, their ability to deplete glutathione, and to produce neoplasia. The effects of elevation of QR1 levels by various enzyme inducers, inhibition of the enzyme by dicumarol, and genetic deletion of the enzyme (knockout mouse) are all consistent with the proposed protective functions. Measurement of QR1 activity in murine hepatoma cells grown in 96-well microtiter plates has provided a rapid and quantitative method for detecting inducer activity and determining inducer potency. This constitutes a strategy for the identification of potential chemoprotectors against cancer. Epidemiological studies show that humans who are genetically deficient in QR1 are more susceptible to the hematological toxicity and carcinogenicity of benzene exposure, and may be more susceptible to the development of a number of malignant tumors. (C) 2000 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)231-240
Number of pages10
JournalFree Radical Biology and Medicine
Volume29
Issue number3-4
DOIs
Publication statusPublished - 1 Aug 2000

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NAD(P)H Dehydrogenase (Quinone)
Quinones
Toxicity
Hydroquinones
Dicumarol
Oxygen
Enzyme inhibition
Enzymes
Benzene
NAD
Glutathione
Tumors
Neoplasms
Cells
Knockout Mice
Oxidation-Reduction
Electrons
Epidemiologic Studies
Hepatocellular Carcinoma

Keywords

  • Antioxidant response element (ARE)
  • Benzene toxicity
  • Chemoprotection
  • Free radicals
  • Hydroquinones
  • Redox cycling
  • Sulforaphane

Cite this

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title = "Persuasive evidence that quinone reductase type 1 (DT diaphorase) protects cells against the toxicity of electrophiles and reactive forms of oxygen",
abstract = "An extensive body of evidence supports the conclusion that by catalyzing obligatory two-electron reductions of quinones to hydroquinones, NAD(P)H:quinone reductase (QR1) protects cells against the deleterious effects of redox cycling of quinones, their ability to deplete glutathione, and to produce neoplasia. The effects of elevation of QR1 levels by various enzyme inducers, inhibition of the enzyme by dicumarol, and genetic deletion of the enzyme (knockout mouse) are all consistent with the proposed protective functions. Measurement of QR1 activity in murine hepatoma cells grown in 96-well microtiter plates has provided a rapid and quantitative method for detecting inducer activity and determining inducer potency. This constitutes a strategy for the identification of potential chemoprotectors against cancer. Epidemiological studies show that humans who are genetically deficient in QR1 are more susceptible to the hematological toxicity and carcinogenicity of benzene exposure, and may be more susceptible to the development of a number of malignant tumors. (C) 2000 Elsevier Science Inc.",
keywords = "Antioxidant response element (ARE), Benzene toxicity, Chemoprotection, Free radicals, Hydroquinones, Redox cycling, Sulforaphane",
author = "Dinkova-Kostova, {Albena T.} and Paul Talalay",
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AU - Dinkova-Kostova, Albena T.

AU - Talalay, Paul

PY - 2000/8/1

Y1 - 2000/8/1

N2 - An extensive body of evidence supports the conclusion that by catalyzing obligatory two-electron reductions of quinones to hydroquinones, NAD(P)H:quinone reductase (QR1) protects cells against the deleterious effects of redox cycling of quinones, their ability to deplete glutathione, and to produce neoplasia. The effects of elevation of QR1 levels by various enzyme inducers, inhibition of the enzyme by dicumarol, and genetic deletion of the enzyme (knockout mouse) are all consistent with the proposed protective functions. Measurement of QR1 activity in murine hepatoma cells grown in 96-well microtiter plates has provided a rapid and quantitative method for detecting inducer activity and determining inducer potency. This constitutes a strategy for the identification of potential chemoprotectors against cancer. Epidemiological studies show that humans who are genetically deficient in QR1 are more susceptible to the hematological toxicity and carcinogenicity of benzene exposure, and may be more susceptible to the development of a number of malignant tumors. (C) 2000 Elsevier Science Inc.

AB - An extensive body of evidence supports the conclusion that by catalyzing obligatory two-electron reductions of quinones to hydroquinones, NAD(P)H:quinone reductase (QR1) protects cells against the deleterious effects of redox cycling of quinones, their ability to deplete glutathione, and to produce neoplasia. The effects of elevation of QR1 levels by various enzyme inducers, inhibition of the enzyme by dicumarol, and genetic deletion of the enzyme (knockout mouse) are all consistent with the proposed protective functions. Measurement of QR1 activity in murine hepatoma cells grown in 96-well microtiter plates has provided a rapid and quantitative method for detecting inducer activity and determining inducer potency. This constitutes a strategy for the identification of potential chemoprotectors against cancer. Epidemiological studies show that humans who are genetically deficient in QR1 are more susceptible to the hematological toxicity and carcinogenicity of benzene exposure, and may be more susceptible to the development of a number of malignant tumors. (C) 2000 Elsevier Science Inc.

KW - Antioxidant response element (ARE)

KW - Benzene toxicity

KW - Chemoprotection

KW - Free radicals

KW - Hydroquinones

KW - Redox cycling

KW - Sulforaphane

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JO - Free Radical Biology and Medicine

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