Perturbations of the actin cytoskeleton activate a Dictyostelium STAT signalling pathway

Tsuyoshi Araki, Jeffrey G Williams

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    The Dictyostelium transcription factor STATc is tyrosine phosphorylated and accumulates in the nucleus when cells are exposed either to hyper-osmotic stress or to the prestalk-inducing polyketide DIF-1. In the case of stress STAT activation is mediated by regulated dephosphorylation; whereby two serine residues on PTP3, the tyrosine phosphatase that de-activates STATc, become phosphorylated after exposure to stress so inhibiting enzymatic activity. We now show that the more highly regulated of the two PTP3 serine residues, S747, is also phosphorylated in response to DIF-1, suggesting a common activation mechanism. Hyper-osmotic stress causes a re-distribution of F-actin to the cortex, cell rounding and shrinkage and we show that DIF-1 induces a similar but transient F-actin re-distribution and rounding response. We also find that two mechanistically distinct inhibitors of actin polymerization, latrunculin A and cytochalasin A induce phosphorylation at S747 of PTP3 and activate STATc. We suggest that PTP3 phosphorylation, and consequent STATc activation, are regulated by changes in F-actin polymerization status during stress and DIF-induced cytoskeletal remodelling.
    Original languageEnglish
    Pages (from-to)420-425
    Number of pages6
    JournalEuropean Journal of Cell Biology
    Volume91
    Issue number5
    DOIs
    Publication statusPublished - 1 May 2012

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    Dictyostelium
    Actin Cytoskeleton
    Actins
    Osmotic Pressure
    Polymerization
    Serine
    Tyrosine
    Phosphorylation
    Polyketides
    Cell Nucleus
    Phosphoric Monoester Hydrolases
    Transcription Factors

    Cite this

    Araki, Tsuyoshi ; Williams, Jeffrey G. / Perturbations of the actin cytoskeleton activate a Dictyostelium STAT signalling pathway. In: European Journal of Cell Biology. 2012 ; Vol. 91, No. 5. pp. 420-425.
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    Perturbations of the actin cytoskeleton activate a Dictyostelium STAT signalling pathway. / Araki, Tsuyoshi; Williams, Jeffrey G.

    In: European Journal of Cell Biology, Vol. 91, No. 5, 01.05.2012, p. 420-425.

    Research output: Contribution to journalArticle

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