Phenotypical and molecular characterization of the Tomato mottle Taino virus-Nicotiana megalosiphon interaction

Cyrelys Collazo; Pedro Luis Ramos; Osmany Chacón; Carlos Javier Borroto; Yunior López; Merardo Pujol; Bart P H J Thomma; Ingo Hein; Orlando Borrás-Hidalgo

doi:10.1016/j.pmpp.2006.02.003

Phenotypical and molecular characterization of the Tomato mottle Taino virus-Nicotiana megalosiphon interaction

Cyrelys Collazo, Pedro Luis Ramos, Osmany Chacón, Carlos Javier Borroto, Yunior López, Merardo Pujol, Bart P H J Thomma, Ingo Hein, Orlando Borrás-Hidalgo

Plant Sciences

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Tomato mottle Taino virus (ToMoTV) infection causes significant yield losses in plants of various Solanaceous species. In this study, the interaction between Nicotiana megalosiphon and ToMoTV was characterized on a phenotypical and molecular basis. In order to isolate genes that are differentially expressed during the interaction of N. megalosiphon with ToMoTV, a PCR-based suppression subtractive hybridization (SSH) was utilized. RNA dot-blot analysis confirmed induction of representative genes upon ToMoTV inoculation at different time points. Interestingly, most of the genes identified are reported here for the first time to be involved in the response of N. megalosiphon to begomovirus infection.

Original language	English
Pages (from-to)	231-236
Number of pages	6
Journal	Physiological and Molecular Plant Pathology
Volume	67
Issue number	3-5
DOIs	https://doi.org/10.1016/j.pmpp.2006.02.003
Publication status	Published - Apr 2006

Keywords

Begomovirus
Nicotiana megalosiphon
Plant defense
SSH
Suppression subtractive hybridization
ToMoTV

ASJC Scopus subject areas

Genetics
Plant Science

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10.1016/j.pmpp.2006.02.003

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title = "Phenotypical and molecular characterization of the Tomato mottle Taino virus-Nicotiana megalosiphon interaction",

abstract = "Tomato mottle Taino virus (ToMoTV) infection causes significant yield losses in plants of various Solanaceous species. In this study, the interaction between Nicotiana megalosiphon and ToMoTV was characterized on a phenotypical and molecular basis. In order to isolate genes that are differentially expressed during the interaction of N. megalosiphon with ToMoTV, a PCR-based suppression subtractive hybridization (SSH) was utilized. RNA dot-blot analysis confirmed induction of representative genes upon ToMoTV inoculation at different time points. Interestingly, most of the genes identified are reported here for the first time to be involved in the response of N. megalosiphon to begomovirus infection.",

keywords = "Begomovirus, Nicotiana megalosiphon, Plant defense, SSH, Suppression subtractive hybridization, ToMoTV",

author = "Cyrelys Collazo and Ramos, {Pedro Luis} and Osmany Chac{\'o}n and Borroto, {Carlos Javier} and Yunior L{\'o}pez and Merardo Pujol and Thomma, {Bart P H J} and Ingo Hein and Orlando Borr{\'a}s-Hidalgo",

year = "2006",

month = apr,

doi = "10.1016/j.pmpp.2006.02.003",

language = "English",

volume = "67",

pages = "231--236",

journal = "Physiological and Molecular Plant Pathology",

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T1 - Phenotypical and molecular characterization of the Tomato mottle Taino virus-Nicotiana megalosiphon interaction

AU - Collazo, Cyrelys

AU - Ramos, Pedro Luis

AU - Chacón, Osmany

AU - Borroto, Carlos Javier

AU - López, Yunior

AU - Pujol, Merardo

AU - Thomma, Bart P H J

AU - Hein, Ingo

AU - Borrás-Hidalgo, Orlando

PY - 2006/4

Y1 - 2006/4

N2 - Tomato mottle Taino virus (ToMoTV) infection causes significant yield losses in plants of various Solanaceous species. In this study, the interaction between Nicotiana megalosiphon and ToMoTV was characterized on a phenotypical and molecular basis. In order to isolate genes that are differentially expressed during the interaction of N. megalosiphon with ToMoTV, a PCR-based suppression subtractive hybridization (SSH) was utilized. RNA dot-blot analysis confirmed induction of representative genes upon ToMoTV inoculation at different time points. Interestingly, most of the genes identified are reported here for the first time to be involved in the response of N. megalosiphon to begomovirus infection.

AB - Tomato mottle Taino virus (ToMoTV) infection causes significant yield losses in plants of various Solanaceous species. In this study, the interaction between Nicotiana megalosiphon and ToMoTV was characterized on a phenotypical and molecular basis. In order to isolate genes that are differentially expressed during the interaction of N. megalosiphon with ToMoTV, a PCR-based suppression subtractive hybridization (SSH) was utilized. RNA dot-blot analysis confirmed induction of representative genes upon ToMoTV inoculation at different time points. Interestingly, most of the genes identified are reported here for the first time to be involved in the response of N. megalosiphon to begomovirus infection.

KW - Begomovirus

KW - Nicotiana megalosiphon

KW - Plant defense

KW - SSH

KW - Suppression subtractive hybridization

KW - ToMoTV

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DO - 10.1016/j.pmpp.2006.02.003

M3 - Article

AN - SCOPUS:33744519358

SN - 0885-5765

VL - 67

SP - 231

EP - 236

JO - Physiological and Molecular Plant Pathology

JF - Physiological and Molecular Plant Pathology

IS - 3-5

ER -

Phenotypical and molecular characterization of the Tomato mottle Taino virus-Nicotiana megalosiphon interaction

Abstract

Keywords

ASJC Scopus subject areas

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