Phorbol esters regulate CD2- and CD3-mediated calcium responses in peripheral blood-derived human T cells

D. A. Cantrell (Lead / Corresponding author), S. C. Lucas, S. Ward, J. Westwick, M. Gullberg

    Research output: Contribution to journalArticle

    15 Citations (Scopus)

    Abstract

    The purpose of the present study was to examine the effect of protein kinase C (pkC) activation on calcium responses generated through the CD3 and CD2 Ag in both normal peripheral blood-derived T lymphocytes and the leukemic T cell line Jurkat. The data reveal a major difference with respect to the regulation of receptor-mediated calcium responses in these two cells. Thus, the pkC activator phorbol-12,13-dibutyrate (Pdbu) enhances calcium responses induced via CD3 and CD2 molecules in normal T cells by accelerating the rate of elevation of intracellular calcium levels and increasing the maximum change in calcium concentration achieved. In contrast, Pdbu inhibits both CD3- and CD2-induced calcium responses in Jurkat cells. Pdbu does not influence calcium responses generated by the guanine nucleotide-binding protein activator, aluminum fluoride, indicating that the effect of pkC occurs at a point proximal to a guanine nucleotide-binding protein regulation of T cell calcium responses.

    Original languageEnglish
    Pages (from-to)3653-3658
    Number of pages6
    JournalJournal of Immunology
    Volume143
    Issue number11
    Publication statusPublished - 1 Dec 1989

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