Phorbol esters regulate CD2- and CD3-mediated calcium responses in peripheral blood-derived human T cells

TY - JOUR

T1 - Phorbol esters regulate CD2- and CD3-mediated calcium responses in peripheral blood-derived human T cells

AU - Cantrell, D. A.

AU - Lucas, S. C.

AU - Ward, S.

AU - Westwick, J.

AU - Gullberg, M.

PY - 1989/12/1

Y1 - 1989/12/1

N2 - The purpose of the present study was to examine the effect of protein kinase C (pkC) activation on calcium responses generated through the CD3 and CD2 Ag in both normal peripheral blood-derived T lymphocytes and the leukemic T cell line Jurkat. The data reveal a major difference with respect to the regulation of receptor-mediated calcium responses in these two cells. Thus, the pkC activator phorbol-12,13-dibutyrate (Pdbu) enhances calcium responses induced via CD3 and CD2 molecules in normal T cells by accelerating the rate of elevation of intracellular calcium levels and increasing the maximum change in calcium concentration achieved. In contrast, Pdbu inhibits both CD3- and CD2-induced calcium responses in Jurkat cells. Pdbu does not influence calcium responses generated by the guanine nucleotide-binding protein activator, aluminum fluoride, indicating that the effect of pkC occurs at a point proximal to a guanine nucleotide-binding protein regulation of T cell calcium responses.

AB - The purpose of the present study was to examine the effect of protein kinase C (pkC) activation on calcium responses generated through the CD3 and CD2 Ag in both normal peripheral blood-derived T lymphocytes and the leukemic T cell line Jurkat. The data reveal a major difference with respect to the regulation of receptor-mediated calcium responses in these two cells. Thus, the pkC activator phorbol-12,13-dibutyrate (Pdbu) enhances calcium responses induced via CD3 and CD2 molecules in normal T cells by accelerating the rate of elevation of intracellular calcium levels and increasing the maximum change in calcium concentration achieved. In contrast, Pdbu inhibits both CD3- and CD2-induced calcium responses in Jurkat cells. Pdbu does not influence calcium responses generated by the guanine nucleotide-binding protein activator, aluminum fluoride, indicating that the effect of pkC occurs at a point proximal to a guanine nucleotide-binding protein regulation of T cell calcium responses.

UR - https://www.scopus.com/pages/publications/0024326855

M3 - Article

C2 - 2573636

AN - SCOPUS:0024326855

SN - 0022-1767

VL - 143

SP - 3653

EP - 3658

JO - Journal of Immunology

JF - Journal of Immunology

IS - 11

ER -