Phosphoinositide-dependent kinase 1 controls migration and malignant transformation but not cell growth and proliferation in PTEN-null lymphocytes

David K. Finlay, Linda V. Sinclair, Carmen Feijoo, Caryll M. Waugh, Thijs J. Hagenbeek, Hergen Spits, Doreen A. Cantrell (Lead / Corresponding author)

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    Abstract

    In normal T cell progenitors, phosphoinositide-dependent kinase l (PDK1)-mediated phosphorylation and activation of protein kinase B (PKB) is essential for the phosphorylation and inactivation of Foxo family transcription factors, and also controls T cell growth and proliferation. The current study has characterized the role of PDK1 in the pathology caused by deletion of the tumor suppressor phosphatase and tensin homologue deleted on chromosome 10 (PTEN). PDK1 is shown to be essential for lymphomagenesis caused by deletion of PTEN in T cell progenitors. However, PTEN deletion bypasses the normal PDK1-controlled signaling pathways that determine thymocyte growth and proliferation. PDK1 does have important functions in PTEN-null thymocytes, notably to control the PKB-Foxo signaling axis and to direct the repertoire of adhesion and chemokine receptors expressed by PTEN-null T cells. The results thus provide two novel insights concerning pathological signaling caused by PTEN loss in lymphocytes. First, PTEN deletion bypasses the normal PDK1-controlled metabolic checkpoints that determine cell growth and proliferation. Second, PDK1 determines the cohort of chemokine and adhesion receptors expressed by PTEN-null cells, thereby controlling their migratory capacity.

    Original languageEnglish
    Pages (from-to)2441-2454
    Number of pages14
    JournalJournal of Experimental Medicine
    Volume206
    Issue number11
    DOIs
    Publication statusPublished - 26 Oct 2009

    Keywords

    • Secondary lymphoid organs
    • Leukemic stem cells
    • Naive T-cells
    • L-selectin
    • Phosphatidylinositol 3-kinase
    • Deficient mice
    • Tumorigenesis
    • Cancer
    • Requirement
    • Activation

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