Phosphorylation by cyclin B-Cdk underlies release of mitotic exit activator Cdc14 from the nucleolus

Ramzi Azzam; Susan L. Chen; Wenying Shou; Angie S. Mah; Gabriela Alexandru; Kim Nasmyth; Roland S. Annan; Steven A. Carr; Raymond J. Deshaies

doi:10.1126/science.1099402

Phosphorylation by cyclin B-Cdk underlies release of mitotic exit activator Cdc14 from the nucleolus

Ramzi Azzam, Susan L. Chen, Wenying Shou, Angie S. Mah, Gabriela Alexandru, Kim Nasmyth, Roland S. Annan, Steven A. Carr, Raymond J. Deshaies

Research output: Contribution to journal › Article › peer-review

153 Citations (Scopus)

Abstract

Budding yeast protein phosphatase Cdc14 is sequestered in the nucleolus in an inactive state during interphase by the anchor protein Net1. Upon entry into anaphase, the Cdc14 early anaphase release ( FEAR) network initiates dispersal of active Cdc14 throughout the cell. We report that the FEAR network promotes phosphorylation of Net1 by cyclin-dependent kinase (Cdk) complexed with cyclin B1 or cyclin B2. These phosphorylations appear to be required for FEAR and sustain the proper timing of late mitotic events. Thus, a regulatory circuit exists to ensure that the arbiter of the mitotic state, Cdk, sets in motion events that culminate in exit from mitosis.

Original language	English
Pages (from-to)	516-519
Number of pages	4
Journal	Science
Volume	305
Issue number	5683
DOIs	https://doi.org/10.1126/science.1099402
Publication status	Published - 2004

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title = "Phosphorylation by cyclin B-Cdk underlies release of mitotic exit activator Cdc14 from the nucleolus",

abstract = "Budding yeast protein phosphatase Cdc14 is sequestered in the nucleolus in an inactive state during interphase by the anchor protein Net1. Upon entry into anaphase, the Cdc14 early anaphase release ( FEAR) network initiates dispersal of active Cdc14 throughout the cell. We report that the FEAR network promotes phosphorylation of Net1 by cyclin-dependent kinase (Cdk) complexed with cyclin B1 or cyclin B2. These phosphorylations appear to be required for FEAR and sustain the proper timing of late mitotic events. Thus, a regulatory circuit exists to ensure that the arbiter of the mitotic state, Cdk, sets in motion events that culminate in exit from mitosis.",

author = "Ramzi Azzam and Chen, {Susan L.} and Wenying Shou and Mah, {Angie S.} and Gabriela Alexandru and Kim Nasmyth and Annan, {Roland S.} and Carr, {Steven A.} and Deshaies, {Raymond J.}",

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doi = "10.1126/science.1099402",

language = "English",

volume = "305",

pages = "516--519",

journal = "Science",

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publisher = "American Association for the Advancement of Science",

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TY - JOUR

T1 - Phosphorylation by cyclin B-Cdk underlies release of mitotic exit activator Cdc14 from the nucleolus

AU - Azzam, Ramzi

AU - Chen, Susan L.

AU - Shou, Wenying

AU - Mah, Angie S.

AU - Alexandru, Gabriela

AU - Nasmyth, Kim

AU - Annan, Roland S.

AU - Carr, Steven A.

AU - Deshaies, Raymond J.

PY - 2004

Y1 - 2004

N2 - Budding yeast protein phosphatase Cdc14 is sequestered in the nucleolus in an inactive state during interphase by the anchor protein Net1. Upon entry into anaphase, the Cdc14 early anaphase release ( FEAR) network initiates dispersal of active Cdc14 throughout the cell. We report that the FEAR network promotes phosphorylation of Net1 by cyclin-dependent kinase (Cdk) complexed with cyclin B1 or cyclin B2. These phosphorylations appear to be required for FEAR and sustain the proper timing of late mitotic events. Thus, a regulatory circuit exists to ensure that the arbiter of the mitotic state, Cdk, sets in motion events that culminate in exit from mitosis.

AB - Budding yeast protein phosphatase Cdc14 is sequestered in the nucleolus in an inactive state during interphase by the anchor protein Net1. Upon entry into anaphase, the Cdc14 early anaphase release ( FEAR) network initiates dispersal of active Cdc14 throughout the cell. We report that the FEAR network promotes phosphorylation of Net1 by cyclin-dependent kinase (Cdk) complexed with cyclin B1 or cyclin B2. These phosphorylations appear to be required for FEAR and sustain the proper timing of late mitotic events. Thus, a regulatory circuit exists to ensure that the arbiter of the mitotic state, Cdk, sets in motion events that culminate in exit from mitosis.

U2 - 10.1126/science.1099402

DO - 10.1126/science.1099402

M3 - Article

SN - 0036-8075

VL - 305

SP - 516

EP - 519

JO - Science

JF - Science

IS - 5683

ER -

Phosphorylation by cyclin B-Cdk underlies release of mitotic exit activator Cdc14 from the nucleolus

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