Phosphorylation-dependent interactions between enzymes of plant metabolism and 14-3-3 proteins

Greg Moorhead, Pauline Douglas, Valerie Cotelle, Jean Harthill, Nick Morrice, Sarah Meek, Uta Deiting, Mark Stitt, Marie Scarabel, Alastair Aitken, Carol MacKintosh

    Research output: Contribution to journalArticlepeer-review

    229 Citations (Scopus)

    Abstract

    Far-Western overlays of soluble extracts of cauliflower revealed many proteins that bound to digoxygenin (DIG)labelled 14-3-3 proteins. Binding to DIG-14-3-3s was prevented by prior dephosphorylation of the extract proteins or by competition with 14-3-3-binding phosphopeptides, indicating that the 14-3-3 proteins bind to phosphorylated sites. The proteins that bound to the DIG-14-3-3s were also immunoprecipitated from extracts with anti-14-3-3 antibodies, demonstrating that they were bound to endogenous plant 14-3-3 proteins. 14-3-3-binding proteins were purified from cauliflower extracts, in sufficient quantity for amino acid sequence analysis, by affinity chromatography on immobilised 14-3-3 proteins and specific elution with a 14-3-3-binding phosphopeptide. Purified 14-3-3-binding proteins included sucrose-phosphate synthase, trehalose-6-phosphate synthase, glutamine synthetases, a protein (LIM17) that has been implicated in early floral development, an approximately 20 kDa protein whose mRNA is induced by NaCl, and a calcium-dependent protein kinase that was capable of phosphorylating and rendering nitrate reductase (NR) sensitive to inhibition by 14-3-3 proteins. In contrast to the phosphorylated NR-14-3-3 complex which is activated by dissociation with 14-3-3-binding phosphopeptides, the total sugar-phosphate synthase activity in plant extracts was inhibited by up to 40% by a 14-3-3-binding phosphopeptide and the phosphopeptide-inhibited activity was reactivated by adding excess 14-3-3 proteins. Thus, 14-3-3 proteins are implicated in regulating several aspects of primary N and C metabolism. The procedures described here will be valuable for determining how the phosphorylation and 14-3-3-binding status of defined target proteins change in response to extracellular stimuli.

    Original languageEnglish
    Pages (from-to)1-12
    Number of pages12
    JournalPlant Journal
    Volume18
    Issue number1
    DOIs
    Publication statusPublished - 1999

    Fingerprint

    Dive into the research topics of 'Phosphorylation-dependent interactions between enzymes of plant metabolism and 14-3-3 proteins'. Together they form a unique fingerprint.

    Cite this