Phosphorylation of the leukocyte function-associated antigen-1 (LFA-1) integrin ß2-chain on Thr-758 occurs after T cell receptor stimulation and leads to 14-3-3 recruitment to the integrin, actin cytoskeleton reorganization, and increased adhesion. Here, we have investigated the signaling effects of ß2 integrin Thr-758 phosphorylation. A penetratin-coupled phospho-Thr-758-ß2 peptide (mimicking the part of the integrin ß-chain surrounding Thr-758) stimulated adhesion of human T cells to the LFA-1 ligand intercellular adhesion molecule-1 (ICAM-1). Additionally, the peptide activated the small GTPases Rac-1 and Cdc42 in T cells. Constitutively active forms of Rac-1 and Cdc42, but not Rho, could compensate for the reduction of cell adhesion to ICAM-1 caused by the T758A mutation in the ß2 integrin. Additionally, the active GTPases salvaged the cell-spreading defect of T758A integrin-transfected cells on coated ICAM-1. A dominant negative form of Cdc42, on the other hand, significantly reduced wild-type ß2 integrin-mediated cell adhesion and spreading. In a T cell stimulation system, the pThr-758 penetratin peptide acted in a similar manner to coated ICAM-1 to increase T cell receptor-induced CD69 expression. These results show that Thr-758-phosphorylated LFA-1 is upstream of Rac-1/Cdc42, cell adhesion, and costimulatory activation of human T cells, thus identifying phosphorylation of Thr-758 in ß2 as a proximal element in LFA-1 signaling.© 2007 by The American Society for Biochemistry and Molecular Biology, Inc.
- Actin cytoskeleton reorganization
- Cell stimulation systems
- Costimulatory activation
- Cell adhesion
- Computer simulation
Nurmi, S. M., Autero, M., Raunio, A. K., Gahmberg, C. G., & Fagerholm, S. C. (2007). Phosphorylation of the LFA-1 integrin beta2-chain on Thr-758 leads to adhesion, Rac-1/Cdc42 activation, and stimulation of CD69 expression in human T cells. Journal of Biological Chemistry, 282(2), 968-975. https://doi.org/10.1074/jbc.M608524200