Phytophthora infestans effector AVRblb2 prevents secretion of a plant immune protease at the haustorial interface

Tolga O. Bozkurt, Sebastian Schornack, Joe Win, Takayuki Shindo, Muhammad Ilyas, Ricardo Oliva, Liliana M. Cano, Alexandra M. E. Jones, Edgar Huitema, Renier A. L. van der Hoorn, Sophien Kamoun

    Research output: Contribution to journalArticlepeer-review

    177 Citations (Scopus)

    Abstract

    In response to pathogen attack, plant cells secrete antimicrobial molecules at the site of infection. However, how plant pathogens interfere with defense-related focal secretion remains poorly known. Here we show that the host-translocated RXLR-type effector protein AVRblb2 of the Irish potato famine pathogen Phytophthora infestans focally accumulates around haustoria, specialized infection structures that form inside plant cells, and promotes virulence by interfering with the execution of host defenses. AVRblb2 significantly enhances susceptibility of host plants to P. infestans by targeting the host papain-like cysteine protease C14 and specifically preventing its secretion into the apoplast. Plants altered in C14 expression were significantly affected in susceptibility to P. infestans in a manner consistent with a positive role of C14 in plant immunity. Our findings point to a unique counterdefense strategy that plant pathogens use to neutralize secreted host defense proteases. Effectors, such as AVRblb2, can be used as molecular probes to dissect focal immune responses at pathogen penetration sites.

    Original languageEnglish
    Pages (from-to)20832-20837
    Number of pages6
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume108
    Issue number51
    DOIs
    Publication statusPublished - 20 Dec 2011

    Keywords

    • plant cell-autonomous immunity
    • polarized secretion
    • late blight
    • CYSTEINE PROTEASE
    • HOST-PLANT
    • ARABIDOPSIS
    • DEFENSE
    • TOMATO
    • RESISTANCE
    • PATHOGENESIS
    • CYTOSKELETON
    • PROTEINASES
    • EXPRESSION

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