Projects per year
Abstract
The convergence of two DNA replication forks creates unique problems during DNA replication termination. In E. coli and SV40, the release of torsional strain by type II topoisomerases is critical for converging replisomes to complete DNA synthesis, but the pathways that mediate fork convergence in eukaryotes are unknown. We studied the convergence of reconstituted yeast replication forks that include all core replisome components and both type I and type II topoisomerases. We found that most converging forks stall at a very late stage, indicating a role for additional factors. We showed that the Pif1 and Rrm3 DNA helicases promote efficient fork convergence and completion of DNA synthesis, even in the absence of type II topoisomerase. Furthermore, Rrm3 and Pif1 are also important for termination of plasmid DNA replication in vivo. These findings identify a eukaryotic pathway for DNA replication termination that is distinct from previously characterized prokaryotic mechanisms.
Original language | English |
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Pages (from-to) | 231-244.e9 |
Number of pages | 24 |
Journal | Molecular Cell |
Volume | 74 |
Issue number | 2 |
Early online date | 5 Mar 2019 |
DOIs | |
Publication status | Published - 18 Apr 2019 |
Keywords
- CMG helicase
- DNA replication termination
- Pif1
- Rrm3
- Top2
- chromosome replication
- fork convergence
- replisome
- topoisomerase
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology
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Dive into the research topics of 'Pif1-family helicases support fork convergence during DNA replication termination in eukaryotes'. Together they form a unique fingerprint.Projects
- 2 Finished
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Molecular Mechanisms of DNA Replication Termination
Deegan, T. (Investigator)
1/05/17 → 1/11/21
Project: Research
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Functional Dissection of the Eukaryotic Replisome (Senior Investigator Award)
Labib, K. (Investigator)
1/04/14 → 29/02/20
Project: Research