(+)-Pinoresinol/(+)-lariciresinol reductase from Forsythia intermedia: Protein purification, cDNA cloning, heterologous expression and comparison to isoflavone reductase

Albena T. Dinkova-Kostova, David R. Gang, Laurence B. Davin, Diana L. Bedgar, Alex Chu, Norman G. Lewis

Research output: Contribution to journalArticle

156 Citations (Scopus)

Abstract

Lignans are a widely distributed class of natural products, whose functions and distribution suggest that they are one of the earliest forms of defense to have evolved in vascular plants; some, such as podophyllotoxin and enterodiol, have important roles in cancer chemotherapy and prevention, respectively. Entry into lignan enzymology has been gained by the ~3000- fold purification of two isoforms of (+)-pinoresinol/(+)-lariciresinol reductase, a pivotal branchpoint enzyme in lignan biosynthesis. Both have comparable (~34.9 kDa) molecular mass and kinetic (V(max)/K(m)) properties and catalyze sequential, NADPH-dependent, stereospecific, hydride transfers where the incoming hydride takes up the pro-R position. The gene encoding (+)-pinoresinol/(+)-lariciresinol reductase has been cloned and the recombinant protein heterologously expressed as a functional β- galactosidase fusion protein. Its amino acid sequence reveals a strong homology to isoflavone reductase, a key branchpoint enzyme in isoflavonoid metabolism and primarily found in the Fabaceae (angiosperms). This is of great evolutionary significance since both lignans and isoflavonoids have comparable plant defense properties, as well as similar roles as phytoestrogens. Given that lignans are widespread from primitive plants onwards, whereas the isoflavone reductase-derived isoflavonoids are mainly restricted to the Fabaceae, it is tempting to speculate that this branch of the isoflavonoid pathway arose via evolutionary divergence from that giving the lignans.

Original languageEnglish
Pages (from-to)29473-29482
Number of pages10
JournalJournal of Biological Chemistry
Volume271
Issue number46
DOIs
Publication statusPublished - 26 Nov 1996

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vestitone reductase
Forsythia
Lignans
Cloning
Purification
Organism Cloning
Complementary DNA
Proteins
Fabaceae
Hydrides
Galactosidases
Podophyllotoxin
Angiosperms
Phytoestrogens
Gene encoding
Chemotherapy
Biosynthesis
Molecular mass
Enzymes
Biological Products

Cite this

@article{7af0280909034975a98e02ff9222698a,
title = "(+)-Pinoresinol/(+)-lariciresinol reductase from Forsythia intermedia: Protein purification, cDNA cloning, heterologous expression and comparison to isoflavone reductase",
abstract = "Lignans are a widely distributed class of natural products, whose functions and distribution suggest that they are one of the earliest forms of defense to have evolved in vascular plants; some, such as podophyllotoxin and enterodiol, have important roles in cancer chemotherapy and prevention, respectively. Entry into lignan enzymology has been gained by the ~3000- fold purification of two isoforms of (+)-pinoresinol/(+)-lariciresinol reductase, a pivotal branchpoint enzyme in lignan biosynthesis. Both have comparable (~34.9 kDa) molecular mass and kinetic (V(max)/K(m)) properties and catalyze sequential, NADPH-dependent, stereospecific, hydride transfers where the incoming hydride takes up the pro-R position. The gene encoding (+)-pinoresinol/(+)-lariciresinol reductase has been cloned and the recombinant protein heterologously expressed as a functional β- galactosidase fusion protein. Its amino acid sequence reveals a strong homology to isoflavone reductase, a key branchpoint enzyme in isoflavonoid metabolism and primarily found in the Fabaceae (angiosperms). This is of great evolutionary significance since both lignans and isoflavonoids have comparable plant defense properties, as well as similar roles as phytoestrogens. Given that lignans are widespread from primitive plants onwards, whereas the isoflavone reductase-derived isoflavonoids are mainly restricted to the Fabaceae, it is tempting to speculate that this branch of the isoflavonoid pathway arose via evolutionary divergence from that giving the lignans.",
author = "Dinkova-Kostova, {Albena T.} and Gang, {David R.} and Davin, {Laurence B.} and Bedgar, {Diana L.} and Alex Chu and Lewis, {Norman G.}",
year = "1996",
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(+)-Pinoresinol/(+)-lariciresinol reductase from Forsythia intermedia : Protein purification, cDNA cloning, heterologous expression and comparison to isoflavone reductase. / Dinkova-Kostova, Albena T.; Gang, David R.; Davin, Laurence B.; Bedgar, Diana L.; Chu, Alex; Lewis, Norman G.

In: Journal of Biological Chemistry, Vol. 271, No. 46, 26.11.1996, p. 29473-29482.

Research output: Contribution to journalArticle

TY - JOUR

T1 - (+)-Pinoresinol/(+)-lariciresinol reductase from Forsythia intermedia

T2 - Protein purification, cDNA cloning, heterologous expression and comparison to isoflavone reductase

AU - Dinkova-Kostova, Albena T.

AU - Gang, David R.

AU - Davin, Laurence B.

AU - Bedgar, Diana L.

AU - Chu, Alex

AU - Lewis, Norman G.

PY - 1996/11/26

Y1 - 1996/11/26

N2 - Lignans are a widely distributed class of natural products, whose functions and distribution suggest that they are one of the earliest forms of defense to have evolved in vascular plants; some, such as podophyllotoxin and enterodiol, have important roles in cancer chemotherapy and prevention, respectively. Entry into lignan enzymology has been gained by the ~3000- fold purification of two isoforms of (+)-pinoresinol/(+)-lariciresinol reductase, a pivotal branchpoint enzyme in lignan biosynthesis. Both have comparable (~34.9 kDa) molecular mass and kinetic (V(max)/K(m)) properties and catalyze sequential, NADPH-dependent, stereospecific, hydride transfers where the incoming hydride takes up the pro-R position. The gene encoding (+)-pinoresinol/(+)-lariciresinol reductase has been cloned and the recombinant protein heterologously expressed as a functional β- galactosidase fusion protein. Its amino acid sequence reveals a strong homology to isoflavone reductase, a key branchpoint enzyme in isoflavonoid metabolism and primarily found in the Fabaceae (angiosperms). This is of great evolutionary significance since both lignans and isoflavonoids have comparable plant defense properties, as well as similar roles as phytoestrogens. Given that lignans are widespread from primitive plants onwards, whereas the isoflavone reductase-derived isoflavonoids are mainly restricted to the Fabaceae, it is tempting to speculate that this branch of the isoflavonoid pathway arose via evolutionary divergence from that giving the lignans.

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M3 - Article

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