Pinus Susceptibility to Pitch Canker Triggers Specific Physiological Responses in Symptomatic Plants: An Integrated Approach

Joana Amaral, Barbara Correia, Carla António, Ana Margarida Rodrigues, Aurelio Gómez-Cadenas, Luis Valledor, Robert D. Hancock, Artur Alves, Glória Pinto (Lead / Corresponding author)

Research output: Contribution to journalArticle

1 Citation (Scopus)
45 Downloads (Pure)

Abstract

Fusarium circinatum, the causal agent of pine pitch canker (PPC), is an emergent and still understudied risk that threatens Pinus forests worldwide, with potential production and sustainability losses. In order to explore the response of pine species with distinct levels of susceptibility to PPC, we investigated changes in physiology, hormones, specific gene transcripts, and primary metabolism occurring in symptomatic Pinus pinea, Pinus pinaster, and Pinus radiata upon inoculation with F. circinatum. Pinus radiata and P. pinaster exhibiting high and intermediate susceptibility to PPC, respectively, suffered changes in plant water status and photosynthetic impairment. This was associated with sink metabolism induction, a general accumulation of amino acids and overexpression of pathogenesis-related genes. On the other hand, P. pinea exhibited the greatest resistance to PPC and stomatal opening, transpiration increase, and glycerol accumulation were observed in inoculated plants. A stronger induction of pyruvate decarboxylase transcripts and differential hormones regulation were also found for inoculated P. pinea in comparison with the susceptible Pinus species studied. The specific physiological changes reported herein are the first steps to understand the complex Pinus-Fusarium interaction and create tools for the selection of resistant genotypes thus contributing to disease mitigation.

Original languageEnglish
Article number509
Pages (from-to)1-16
Number of pages16
JournalFrontiers in Plant Science
Volume10
DOIs
Publication statusPublished - 24 Apr 2019

Fingerprint

plant response
Pinus
Pinus pinea
Fusarium circinatum
Pinus pinaster
Pinus radiata
hormones
pyruvate decarboxylase
metabolism
Fusarium
glycerol
transpiration
genes
pathogenesis
physiology
amino acids
genotype
water

Keywords

  • Biotic stress response
  • Disease differential susceptibility
  • Forest tree disease
  • Gene expression
  • Plant hormones
  • Plant physiology
  • Plant primary metabolism

Cite this

Amaral, J., Correia, B., António, C., Rodrigues, A. M., Gómez-Cadenas, A., Valledor, L., ... Pinto, G. (2019). Pinus Susceptibility to Pitch Canker Triggers Specific Physiological Responses in Symptomatic Plants: An Integrated Approach. Frontiers in Plant Science, 10, 1-16. [509]. https://doi.org/10.3389/fpls.2019.00509
Amaral, Joana ; Correia, Barbara ; António, Carla ; Rodrigues, Ana Margarida ; Gómez-Cadenas, Aurelio ; Valledor, Luis ; Hancock, Robert D. ; Alves, Artur ; Pinto, Glória. / Pinus Susceptibility to Pitch Canker Triggers Specific Physiological Responses in Symptomatic Plants : An Integrated Approach. In: Frontiers in Plant Science. 2019 ; Vol. 10. pp. 1-16.
@article{a2a13ed463af4fdc8a37562f6a2c902d,
title = "Pinus Susceptibility to Pitch Canker Triggers Specific Physiological Responses in Symptomatic Plants: An Integrated Approach",
abstract = "Fusarium circinatum, the causal agent of pine pitch canker (PPC), is an emergent and still understudied risk that threatens Pinus forests worldwide, with potential production and sustainability losses. In order to explore the response of pine species with distinct levels of susceptibility to PPC, we investigated changes in physiology, hormones, specific gene transcripts, and primary metabolism occurring in symptomatic Pinus pinea, Pinus pinaster, and Pinus radiata upon inoculation with F. circinatum. Pinus radiata and P. pinaster exhibiting high and intermediate susceptibility to PPC, respectively, suffered changes in plant water status and photosynthetic impairment. This was associated with sink metabolism induction, a general accumulation of amino acids and overexpression of pathogenesis-related genes. On the other hand, P. pinea exhibited the greatest resistance to PPC and stomatal opening, transpiration increase, and glycerol accumulation were observed in inoculated plants. A stronger induction of pyruvate decarboxylase transcripts and differential hormones regulation were also found for inoculated P. pinea in comparison with the susceptible Pinus species studied. The specific physiological changes reported herein are the first steps to understand the complex Pinus-Fusarium interaction and create tools for the selection of resistant genotypes thus contributing to disease mitigation.",
keywords = "Biotic stress response, Disease differential susceptibility, Forest tree disease, Gene expression, Plant hormones, Plant physiology, Plant primary metabolism",
author = "Joana Amaral and Barbara Correia and Carla Ant{\'o}nio and Rodrigues, {Ana Margarida} and Aurelio G{\'o}mez-Cadenas and Luis Valledor and Hancock, {Robert D.} and Artur Alves and Gl{\'o}ria Pinto",
note = "This work is a contribution of URGENTpine (PTDC/AGRFOR/2768/2014) funded by FCT – Funda{\cc}{\~a}o para a Ci{\^e}ncia e a Tecnologia, I.P., through national funds, and the co-funding by the FEDER (POCI-01-0145-FEDER-016785), within the PT2020 Partnership Agreement and Compete 2020. Thanks are due for the financial support to CESAM (UID/AMB/50017/2019), to FCT/MCTES through national funds. FCT supported JA (SFRH/BD/120967/2016), AMR (PD/BD/114417/2016), and CA (IF/00376/2012/CP0165/CT0003). GP was funded by national funds (OE), through FCT, in the scope of the framework contract foreseen in numbers 4, 5, and 6 of the article 23, of the Decree-Law 57/2016, of August 29, changed by Law 57/2017, of July 19. This article is based upon work carried out during COST Action FP1406 PINESTRENGTH (PPC-strategies for management of Gibberella circinata in greenhouses and forests), supported by COST (European Cooperation in Science and Technology) that awarded JA with a STSM. CA and AMR acknowledge the ITQB NOVA R&D GREEN-it ‘Bioresources for Sustainability’ (UID/Multi/04551/2013) and International Ph.D. Programme ‘Plants for Life’ (PD/00035/2013), respectively. The James Hutton Institute receives support from the Rural and Environment Science and Analytical Services Division of the Scottish Government.",
year = "2019",
month = "4",
day = "24",
doi = "10.3389/fpls.2019.00509",
language = "English",
volume = "10",
pages = "1--16",
journal = "Frontiers in Plant Science",
issn = "1664-462X",
publisher = "Frontiers Media",

}

Amaral, J, Correia, B, António, C, Rodrigues, AM, Gómez-Cadenas, A, Valledor, L, Hancock, RD, Alves, A & Pinto, G 2019, 'Pinus Susceptibility to Pitch Canker Triggers Specific Physiological Responses in Symptomatic Plants: An Integrated Approach', Frontiers in Plant Science, vol. 10, 509, pp. 1-16. https://doi.org/10.3389/fpls.2019.00509

Pinus Susceptibility to Pitch Canker Triggers Specific Physiological Responses in Symptomatic Plants : An Integrated Approach. / Amaral, Joana; Correia, Barbara; António, Carla; Rodrigues, Ana Margarida; Gómez-Cadenas, Aurelio; Valledor, Luis; Hancock, Robert D.; Alves, Artur; Pinto, Glória (Lead / Corresponding author).

In: Frontiers in Plant Science, Vol. 10, 509, 24.04.2019, p. 1-16.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Pinus Susceptibility to Pitch Canker Triggers Specific Physiological Responses in Symptomatic Plants

T2 - An Integrated Approach

AU - Amaral, Joana

AU - Correia, Barbara

AU - António, Carla

AU - Rodrigues, Ana Margarida

AU - Gómez-Cadenas, Aurelio

AU - Valledor, Luis

AU - Hancock, Robert D.

AU - Alves, Artur

AU - Pinto, Glória

N1 - This work is a contribution of URGENTpine (PTDC/AGRFOR/2768/2014) funded by FCT – Fundação para a Ciência e a Tecnologia, I.P., through national funds, and the co-funding by the FEDER (POCI-01-0145-FEDER-016785), within the PT2020 Partnership Agreement and Compete 2020. Thanks are due for the financial support to CESAM (UID/AMB/50017/2019), to FCT/MCTES through national funds. FCT supported JA (SFRH/BD/120967/2016), AMR (PD/BD/114417/2016), and CA (IF/00376/2012/CP0165/CT0003). GP was funded by national funds (OE), through FCT, in the scope of the framework contract foreseen in numbers 4, 5, and 6 of the article 23, of the Decree-Law 57/2016, of August 29, changed by Law 57/2017, of July 19. This article is based upon work carried out during COST Action FP1406 PINESTRENGTH (PPC-strategies for management of Gibberella circinata in greenhouses and forests), supported by COST (European Cooperation in Science and Technology) that awarded JA with a STSM. CA and AMR acknowledge the ITQB NOVA R&D GREEN-it ‘Bioresources for Sustainability’ (UID/Multi/04551/2013) and International Ph.D. Programme ‘Plants for Life’ (PD/00035/2013), respectively. The James Hutton Institute receives support from the Rural and Environment Science and Analytical Services Division of the Scottish Government.

PY - 2019/4/24

Y1 - 2019/4/24

N2 - Fusarium circinatum, the causal agent of pine pitch canker (PPC), is an emergent and still understudied risk that threatens Pinus forests worldwide, with potential production and sustainability losses. In order to explore the response of pine species with distinct levels of susceptibility to PPC, we investigated changes in physiology, hormones, specific gene transcripts, and primary metabolism occurring in symptomatic Pinus pinea, Pinus pinaster, and Pinus radiata upon inoculation with F. circinatum. Pinus radiata and P. pinaster exhibiting high and intermediate susceptibility to PPC, respectively, suffered changes in plant water status and photosynthetic impairment. This was associated with sink metabolism induction, a general accumulation of amino acids and overexpression of pathogenesis-related genes. On the other hand, P. pinea exhibited the greatest resistance to PPC and stomatal opening, transpiration increase, and glycerol accumulation were observed in inoculated plants. A stronger induction of pyruvate decarboxylase transcripts and differential hormones regulation were also found for inoculated P. pinea in comparison with the susceptible Pinus species studied. The specific physiological changes reported herein are the first steps to understand the complex Pinus-Fusarium interaction and create tools for the selection of resistant genotypes thus contributing to disease mitigation.

AB - Fusarium circinatum, the causal agent of pine pitch canker (PPC), is an emergent and still understudied risk that threatens Pinus forests worldwide, with potential production and sustainability losses. In order to explore the response of pine species with distinct levels of susceptibility to PPC, we investigated changes in physiology, hormones, specific gene transcripts, and primary metabolism occurring in symptomatic Pinus pinea, Pinus pinaster, and Pinus radiata upon inoculation with F. circinatum. Pinus radiata and P. pinaster exhibiting high and intermediate susceptibility to PPC, respectively, suffered changes in plant water status and photosynthetic impairment. This was associated with sink metabolism induction, a general accumulation of amino acids and overexpression of pathogenesis-related genes. On the other hand, P. pinea exhibited the greatest resistance to PPC and stomatal opening, transpiration increase, and glycerol accumulation were observed in inoculated plants. A stronger induction of pyruvate decarboxylase transcripts and differential hormones regulation were also found for inoculated P. pinea in comparison with the susceptible Pinus species studied. The specific physiological changes reported herein are the first steps to understand the complex Pinus-Fusarium interaction and create tools for the selection of resistant genotypes thus contributing to disease mitigation.

KW - Biotic stress response

KW - Disease differential susceptibility

KW - Forest tree disease

KW - Gene expression

KW - Plant hormones

KW - Plant physiology

KW - Plant primary metabolism

UR - http://www.scopus.com/inward/record.url?scp=85067355000&partnerID=8YFLogxK

U2 - 10.3389/fpls.2019.00509

DO - 10.3389/fpls.2019.00509

M3 - Article

C2 - 31068959

VL - 10

SP - 1

EP - 16

JO - Frontiers in Plant Science

JF - Frontiers in Plant Science

SN - 1664-462X

M1 - 509

ER -