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Abstract
We demonstrate the first planar Airy light-sheet microscope. Fluorescence light-sheet microscopy has become the method of choice to study large biological samples with cellular or sub-cellular resolution. The propagation-invariant Airy beam enables a ten-fold increase in field-of-view with single-photon excitation; however, the characteristic asymmetry of the light-sheet limits its potential for multi-photon excitation. Here we show how a planar light-sheet can be formed from the curved propagation-invariant Airy beam. The resulting symmetric light sheet excites two-photon fluorescence uniformly across an extended field-of-view without the need for deconvolution. We demonstrate the method for rapid two-photon imaging of large volumes of neuronal tissue.
Original language | English |
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Pages (from-to) | 3927-3935 |
Number of pages | 9 |
Journal | Biomedical Optics Express |
Volume | 11 |
Issue number | 7 |
Early online date | 22 Jun 2020 |
DOIs | |
Publication status | Published - 1 Jul 2020 |
Keywords
- physics
- optics
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
- Biotechnology
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Correlative Refractive Index Light-Sheet Microscopy
Vettenburg, T. (Investigator)
1/01/20 → 1/07/27
Project: Research