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We demonstrate the first planar Airy light-sheet microscope. Fluorescence light-sheet microscopy has become the method of choice to study large biological samples with cellular or sub-cellular resolution. The propagation-invariant Airy beam enables a ten-fold increase in field-of-view with single-photon excitation; however, the characteristic asymmetry of the light-sheet limits its potential for multi-photon excitation. Here we show how a planar light-sheet can be formed from the curved propagation-invariant Airy beam. The resulting symmetric light sheet excites two-photon fluorescence uniformly across an extended field-of-view without the need for deconvolution. We demonstrate the method for rapid two-photon imaging of large volumes of neuronal tissue.
|Number of pages||9|
|Journal||Biomedical Optics Express|
|Early online date||22 Jun 2020|
|Publication status||Published - 1 Jul 2020|
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
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- 1 Active
Correlative Refractive Index Light-Sheet Microscopy
1/01/20 → 31/12/23