Planar Airy beam light-sheet for two-photon microscopy

TY - JOUR

T1 - Planar Airy beam light-sheet for two-photon microscopy

AU - Hosny, Neveen A.

AU - Seyforth, James A.

AU - Spickermann, Gunnar

AU - Mitchell, Thomas J.

AU - Almada, Pedro

AU - Chesters, Robert

AU - Mitchell, Scott J.

AU - Chennell, George

AU - Vernon, Anthony C.

AU - Cho, Kwangwook

AU - Srivastava, Deepak P.

AU - Forster, Robert

AU - Vettenburg, Tom

N1 - Funding: MRC (MR/S034900/1)

PY - 2020/7/1

Y1 - 2020/7/1

N2 - We demonstrate the first planar Airy light-sheet microscope. Fluorescence light-sheet microscopy has become the method of choice to study large biological samples with cellular or sub-cellular resolution. The propagation-invariant Airy beam enables a ten-fold increase in field-of-view with single-photon excitation; however, the characteristic asymmetry of the light-sheet limits its potential for multi-photon excitation. Here we show how a planar light-sheet can be formed from the curved propagation-invariant Airy beam. The resulting symmetric light sheet excites two-photon fluorescence uniformly across an extended field-of-view without the need for deconvolution. We demonstrate the method for rapid two-photon imaging of large volumes of neuronal tissue.

AB - We demonstrate the first planar Airy light-sheet microscope. Fluorescence light-sheet microscopy has become the method of choice to study large biological samples with cellular or sub-cellular resolution. The propagation-invariant Airy beam enables a ten-fold increase in field-of-view with single-photon excitation; however, the characteristic asymmetry of the light-sheet limits its potential for multi-photon excitation. Here we show how a planar light-sheet can be formed from the curved propagation-invariant Airy beam. The resulting symmetric light sheet excites two-photon fluorescence uniformly across an extended field-of-view without the need for deconvolution. We demonstrate the method for rapid two-photon imaging of large volumes of neuronal tissue.

KW - physics

KW - optics

UR - https://arxiv.org/abs/2001.06829

UR - https://www.scopus.com/pages/publications/85087986128

U2 - 10.1364/BOE.395547

DO - 10.1364/BOE.395547

M3 - Article

C2 - 33014576

SN - 2156-7085

VL - 11

SP - 3927

EP - 3935

JO - Biomedical Optics Express

JF - Biomedical Optics Express

IS - 7

ER -