Planar light-sheet microscopy with curved Airy beams

Tom Vettenburg

doi:10.1117/12.3007903

Planar light-sheet microscopy with curved Airy beams

Tom Vettenburg

Physics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

60 Downloads (Pure)

Abstract

Light-sheet microscopy enables rapid 3D imaging of biological samples. Its field-of-view can be extended ten-fold by relying on propagation-invariant Airy beams. However, such beams propagate on a parabolic trajectory. By consequence, a light-sheet formed by Airy beams is not planar, thus warping the images. Here, we demonstrate a planar Airy light-sheet that does not rely on digital image restoration techniques for two-photon microscopy.

Original language	English
Title of host publication	Biomedical Imaging and Sensing Conference
Editors	Toyohiko Yatagai, Yoshihisa Aizu, Osamu Matoba, Yasuhiro Awatsuji, Yuan Luo
Publisher	Society of Photo-optical Instrumentation Engineers
Number of pages	3
Volume	12608
ISBN (Electronic)	9781510663435
DOIs	https://doi.org/10.1117/12.3007903
Publication status	Published - 20 Sept 2023

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	12608
ISSN (Print)	0277-786X
ISSN (Electronic)	1996-756X

Keywords

Beam shaping
Fluorescence Light-sheet Microscopy
Propagation-invariant beams

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Applied Mathematics
Electrical and Electronic Engineering
Computer Science Applications

Access to Document

10.1117/12.3007903

Author Accepted Manuscript Accepted author manuscript, 413 KBLicence: CC BY

Correlative Refractive Index Light-Sheet Microscopy
Vettenburg, T. (Investigator)
Medical Research Council
1/01/20 → 1/07/27
Project: Research

Cite this

Vettenburg, T. (2023). Planar light-sheet microscopy with curved Airy beams. In T. Yatagai, Y. Aizu, O. Matoba, Y. Awatsuji, & Y. Luo (Eds.), Biomedical Imaging and Sensing Conference (Vol. 12608). Article 126080P (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 12608). Society of Photo-optical Instrumentation Engineers. https://doi.org/10.1117/12.3007903

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abstract = "Light-sheet microscopy enables rapid 3D imaging of biological samples. Its field-of-view can be extended ten-fold by relying on propagation-invariant Airy beams. However, such beams propagate on a parabolic trajectory. By consequence, a light-sheet formed by Airy beams is not planar, thus warping the images. Here, we demonstrate a planar Airy light-sheet that does not rely on digital image restoration techniques for two-photon microscopy.",

keywords = "Beam shaping, Fluorescence Light-sheet Microscopy, Propagation-invariant beams",

author = "Tom Vettenburg",

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publisher = "Society of Photo-optical Instrumentation Engineers",

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Planar light-sheet microscopy with curved Airy beams. / Vettenburg, Tom.
Biomedical Imaging and Sensing Conference. ed. / Toyohiko Yatagai; Yoshihisa Aizu; Osamu Matoba; Yasuhiro Awatsuji; Yuan Luo. Vol. 12608 Society of Photo-optical Instrumentation Engineers, 2023. 126080P (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 12608).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Planar light-sheet microscopy with curved Airy beams

AU - Vettenburg, Tom

N1 - T. Vettenburg is a UKRI Future Leaders Fellow supported by grant MR/S034900/1.

PY - 2023/9/20

Y1 - 2023/9/20

N2 - Light-sheet microscopy enables rapid 3D imaging of biological samples. Its field-of-view can be extended ten-fold by relying on propagation-invariant Airy beams. However, such beams propagate on a parabolic trajectory. By consequence, a light-sheet formed by Airy beams is not planar, thus warping the images. Here, we demonstrate a planar Airy light-sheet that does not rely on digital image restoration techniques for two-photon microscopy.

AB - Light-sheet microscopy enables rapid 3D imaging of biological samples. Its field-of-view can be extended ten-fold by relying on propagation-invariant Airy beams. However, such beams propagate on a parabolic trajectory. By consequence, a light-sheet formed by Airy beams is not planar, thus warping the images. Here, we demonstrate a planar Airy light-sheet that does not rely on digital image restoration techniques for two-photon microscopy.

KW - Beam shaping

KW - Fluorescence Light-sheet Microscopy

KW - Propagation-invariant beams

U2 - 10.1117/12.3007903

DO - 10.1117/12.3007903

M3 - Conference contribution

VL - 12608

T3 - Proceedings of SPIE - The International Society for Optical Engineering

BT - Biomedical Imaging and Sensing Conference

A2 - Yatagai, Toyohiko

A2 - Aizu, Yoshihisa

A2 - Matoba, Osamu

A2 - Awatsuji, Yasuhiro

A2 - Luo, Yuan

PB - Society of Photo-optical Instrumentation Engineers

ER -

Planar light-sheet microscopy with curved Airy beams

Abstract

Publication series

Keywords

ASJC Scopus subject areas

Access to Document

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Projects

Correlative Refractive Index Light-Sheet Microscopy

Cite this