Abstract
Bioinformatics tools to aid gene and protein sequence analysis have become an integral part of biology in the post-genomic era. Release of the Plasmodium falciparum genome sequence has allowed biologists to define the gene and the predicted protein content as well as their sequences in the parasite. Using p/ and molecular weight as characteristics unique to each protein, we have developed a bioinformatics tool to aid identification of proteins from Plasmodium falciparum. The tool makes use of a Virtual 2-DE generated by plotting all of the proteins from the Plasmodium database on a p/ versus molecular weight scale. Proteins are identified by comparing the position of migration of desired protein spots from an experimental 2-DE and that on a virtual 2-DE. The procedure has been automated in the form of user-friendly software called "Plasmo2D".
Original language | English |
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Pages (from-to) | 2369-2374 |
Number of pages | 6 |
Journal | Journal of Proteome Research |
Volume | 4 |
Issue number | 6 |
Early online date | 18 Oct 2005 |
DOIs | |
Publication status | Published - 1 Dec 2005 |
Keywords
- Bioinformatics
- Heat shock proteins
- Malaria
- Plasmo2D
- Proteomics
ASJC Scopus subject areas
- General Chemistry
- Biochemistry