Polar localization of the MinD protein of Bacillus subtilis and its role in selection of the mid-cell division site

Adele L Marston, Helena B. Thomaides, David H. Edwards, Michaela E. Sharpe, Jeffery Errington

    Research output: Contribution to journalArticle

    271 Citations (Scopus)

    Abstract

    Cell division in rod-shaped bacteria is initiated by formation of a ring of the tubulin-like protein FtsZ at mid-cell. Division site selection is controlled by a conserved division inhibitor MinCD, which prevents aberrant division at the cell poles. The Bacillus subtilis DivIVA protein controls the topological specificity of MinCD action. Here we show that DivIVA is targeted to division sites late in their assembly, after some MinCD-sensitive step requiring FtsZ and other division proteins has been passed. DivIVA then recruits MinD to the division sites preventing another division from taking place near the newly formed cell poles. Sequestration of MinD to the poles also releases the next mid-cell sites for division. Remarkably, this mechanism of DivIVA action is completely different from that of the equivalent protein MinE of Escherichia coli, even though both systems operate via the same division inhibitor MinCD.
    Original languageEnglish
    Pages (from-to)3419-3430
    Number of pages12
    JournalGenes & Development
    Volume12
    Issue number21
    Publication statusPublished - 1 Nov 1998

    Keywords

    • Bacillus subtilis
    • DivIVA
    • Cell division
    • MinD
    • Cytokinesis

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