Polarised light sheet tomography

Sascha Reidt, Daniel O'Brien, Kenneth Wood, Michael MacDonald (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)
281 Downloads (Pure)

Abstract

The various benefits of light sheet microscopy have made it a widely used modality for capturing threedimensional images. It is mostly used for fluorescence imaging, but recently another technique called Light Sheet Tomography solely relying on scattering was presented. The method was successfully applied to imaging of plant roots in transparent soil, but is limited when it comes to more turbid samples. This study presents a Polarised Light Sheet Tomography system and its advantages when imaging in highly scattering turbid media. The experimental configuration is guided by Monte Carlo Radiation Transfer methods, which model the propagation of a polarised light sheet in the sample. Images of both reflecting and absorbing phantoms in a complex collagenous matrix were acquired, and the results for different polarisation configurations are compared. Focus scanning methods were then used to reduce noise and produce three dimensional reconstructions of absorbing targets.
Original languageEnglish
Pages (from-to)11239-11249
JournalOptics Express
Volume24
Issue number10
DOIs
Publication statusPublished - 13 May 2016

Keywords

  • Imaging through turbid media
  • Tomography
  • Three-dimensional microscopy
  • Scattering
  • Polarization

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