Poly(acrylic acid)-capped and dye-loaded graphene oxide-mesoporous silica: A nano-sandwich for two-photon and photoacoustic dual-mode imaging

Kim Truc Nguyen, Sivaramapanicker Sreejith, James Joseph, Tingchao He, Parijat Borah, Eng You Guan, Sun Woh Lye, Handong Sun, Yanli Zhao (Lead / Corresponding author)

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The capability of a 2D hybrid material based on mesoporous silica-coated graphene oxide (GO) for unreported two-photon (2P) and photoacoustic (PA) dual-mode imaging was investigated. Nano-sandwich (NS) was synthesized by growing mesoporous silica on both sides of GO. Human cervical epitheloid Carcinoma (HeLa) cells were seeded in a six-well plate, which contains a cover slip at the bottom. Upon achieving 70% cell coverage on the cover slip, PAA@ NS1was added. After the sample was incubated in humidified atmosphere overnight, the medium was removed, and cells were washed three times with phosphate buffered saline (PBS) and then fixed with formaldehyde at room temperature for 15 min. PA signals generated from the samples were studied using the PA system configured with a tunable nanosecond pulsed laser and a high-frequency single-element immersion ultrasound transducer. The signal contrast observed from the hybrid exhibits deep tissue imaging at depths varying from 4 to 8 mm inside thick tissue models. Thus, the new hybrid demonstrates its excellent capability as a PA contrast agent for deep tissue imaging.

Original languageEnglish
Pages (from-to)1060-1066
Number of pages7
JournalParticle and Particle Systems Characterization
Volume31
Issue number10
Early online date15 May 2014
DOIs
Publication statusPublished - Oct 2014

Keywords

  • dual‐mode imaging
  • graphene oxide
  • mesoporous silica
  • photoacoustic
  • two‐photon

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    Nguyen, K. T., Sreejith, S., Joseph, J., He, T., Borah, P., Guan, E. Y., Lye, S. W., Sun, H., & Zhao, Y. (2014). Poly(acrylic acid)-capped and dye-loaded graphene oxide-mesoporous silica: A nano-sandwich for two-photon and photoacoustic dual-mode imaging. Particle and Particle Systems Characterization, 31(10), 1060-1066. https://doi.org/10.1002/ppsc.201400044