The capability of a 2D hybrid material based on mesoporous silica-coated graphene oxide (GO) for unreported two-photon (2P) and photoacoustic (PA) dual-mode imaging was investigated. Nano-sandwich (NS) was synthesized by growing mesoporous silica on both sides of GO. Human cervical epitheloid Carcinoma (HeLa) cells were seeded in a six-well plate, which contains a cover slip at the bottom. Upon achieving 70% cell coverage on the cover slip, PAA@ NS1was added. After the sample was incubated in humidified atmosphere overnight, the medium was removed, and cells were washed three times with phosphate buffered saline (PBS) and then fixed with formaldehyde at room temperature for 15 min. PA signals generated from the samples were studied using the PA system configured with a tunable nanosecond pulsed laser and a high-frequency single-element immersion ultrasound transducer. The signal contrast observed from the hybrid exhibits deep tissue imaging at depths varying from 4 to 8 mm inside thick tissue models. Thus, the new hybrid demonstrates its excellent capability as a PA contrast agent for deep tissue imaging.
|Number of pages||7|
|Journal||Particle and Particle Systems Characterization|
|Early online date||15 May 2014|
|Publication status||Published - Oct 2014|
- dual‐mode imaging
- graphene oxide
- mesoporous silica