Polyubiquitin Binding to Optineurin Is Required for Optimal Activation of TANK-binding Kinase 1 and Production of Interferon beta

Catherine E. Gleason, Alban Ordureau, Robert Gourlay, J. Simon C. Arthur, Philip Cohen

    Research output: Contribution to journalArticle

    100 Citations (Scopus)

    Abstract

    TANK-binding kinase (TBK1) is essential for transcription of the interferon (IFN) beta gene in response to lipopolysaccharide (LPS) and double-stranded RNA, but the molecular mechanisms that underlie the activation of TBK1 are incompletely understood. Previously, we identified the NF-kappa B essential modulator (NEMO)-related polyubiquitin-binding protein, optineurin (OPTN), as a novel binding partner of TBK1. To determine whether the ubiquitin-binding function of OPTN is involved in regulating TBK1 and IFN beta production, we generated a mouse in which wild-type optineurin was replaced by the polyubiquitin binding-defective mutant, OPTND477N/D477N. In this study, we found that LPS or poly(I:C)-induced TBK1 activity was significantly reduced in bone marrow-derived macrophage (BMDM) from OPTND477N/D477N mice. Consistent with this, the phosphorylation of IFN regulatory factor 3 (IRF3) and the production of IFN beta mRNA and secretion were reduced. Stimulation of BMDMs with LPS triggered the phosphorylation of OPTN, which was reversed by phosphatase treatment and prevented by pharmacological inhibition of both the canonical I kappa B kinases (IKK alpha/beta) and the IKK-related kinases (TBK1/IKK epsilon). In contrast, LPS-stimulated phosphorylation of OPTN(D477N) was markedly reduced in BMDMs from OPTND477N/D477N mice, and inhibition of the canonical IKKs alone prevented phosphorylation, providing further evidence that ubiquitin binding to OPTN contributes to LPS-induced TBK1 activation. TBK1 and IKK beta phosphorylated OPTN preferentially at Ser-177 and Ser-513, respectively, in vitro. In conclusion, our results suggest that OPTN binds to polyubiquity-lated species formed in response to LPS and poly(I:C), enhancing the activation of TBK1 that is required for optimal phosphorylation of IRF3 and production of IFN beta.

    Original languageEnglish
    Pages (from-to)35663-35674
    Number of pages12
    JournalJournal of Biological Chemistry
    Volume286
    Issue number41
    DOIs
    Publication statusPublished - 14 Oct 2011

    Keywords

    • NF-KAPPA-B
    • SIGNALING PATHWAY
    • UBIQUITIN CHAINS
    • VIRAL-INFECTION
    • INNATE IMMUNITY
    • IFN RESPONSES
    • CUTTING EDGE
    • IKK-EPSILON
    • NEMO
    • DOMAIN

    Cite this

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    title = "Polyubiquitin Binding to Optineurin Is Required for Optimal Activation of TANK-binding Kinase 1 and Production of Interferon beta",
    abstract = "TANK-binding kinase (TBK1) is essential for transcription of the interferon (IFN) beta gene in response to lipopolysaccharide (LPS) and double-stranded RNA, but the molecular mechanisms that underlie the activation of TBK1 are incompletely understood. Previously, we identified the NF-kappa B essential modulator (NEMO)-related polyubiquitin-binding protein, optineurin (OPTN), as a novel binding partner of TBK1. To determine whether the ubiquitin-binding function of OPTN is involved in regulating TBK1 and IFN beta production, we generated a mouse in which wild-type optineurin was replaced by the polyubiquitin binding-defective mutant, OPTND477N/D477N. In this study, we found that LPS or poly(I:C)-induced TBK1 activity was significantly reduced in bone marrow-derived macrophage (BMDM) from OPTND477N/D477N mice. Consistent with this, the phosphorylation of IFN regulatory factor 3 (IRF3) and the production of IFN beta mRNA and secretion were reduced. Stimulation of BMDMs with LPS triggered the phosphorylation of OPTN, which was reversed by phosphatase treatment and prevented by pharmacological inhibition of both the canonical I kappa B kinases (IKK alpha/beta) and the IKK-related kinases (TBK1/IKK epsilon). In contrast, LPS-stimulated phosphorylation of OPTN(D477N) was markedly reduced in BMDMs from OPTND477N/D477N mice, and inhibition of the canonical IKKs alone prevented phosphorylation, providing further evidence that ubiquitin binding to OPTN contributes to LPS-induced TBK1 activation. TBK1 and IKK beta phosphorylated OPTN preferentially at Ser-177 and Ser-513, respectively, in vitro. In conclusion, our results suggest that OPTN binds to polyubiquity-lated species formed in response to LPS and poly(I:C), enhancing the activation of TBK1 that is required for optimal phosphorylation of IRF3 and production of IFN beta.",
    keywords = "NF-KAPPA-B, SIGNALING PATHWAY, UBIQUITIN CHAINS, VIRAL-INFECTION, INNATE IMMUNITY, IFN RESPONSES, CUTTING EDGE, IKK-EPSILON, NEMO, DOMAIN",
    author = "Gleason, {Catherine E.} and Alban Ordureau and Robert Gourlay and Arthur, {J. Simon C.} and Philip Cohen",
    year = "2011",
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    language = "English",
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    pages = "35663--35674",
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    Polyubiquitin Binding to Optineurin Is Required for Optimal Activation of TANK-binding Kinase 1 and Production of Interferon beta. / Gleason, Catherine E.; Ordureau, Alban; Gourlay, Robert; Arthur, J. Simon C.; Cohen, Philip.

    In: Journal of Biological Chemistry, Vol. 286, No. 41, 14.10.2011, p. 35663-35674.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Polyubiquitin Binding to Optineurin Is Required for Optimal Activation of TANK-binding Kinase 1 and Production of Interferon beta

    AU - Gleason, Catherine E.

    AU - Ordureau, Alban

    AU - Gourlay, Robert

    AU - Arthur, J. Simon C.

    AU - Cohen, Philip

    PY - 2011/10/14

    Y1 - 2011/10/14

    N2 - TANK-binding kinase (TBK1) is essential for transcription of the interferon (IFN) beta gene in response to lipopolysaccharide (LPS) and double-stranded RNA, but the molecular mechanisms that underlie the activation of TBK1 are incompletely understood. Previously, we identified the NF-kappa B essential modulator (NEMO)-related polyubiquitin-binding protein, optineurin (OPTN), as a novel binding partner of TBK1. To determine whether the ubiquitin-binding function of OPTN is involved in regulating TBK1 and IFN beta production, we generated a mouse in which wild-type optineurin was replaced by the polyubiquitin binding-defective mutant, OPTND477N/D477N. In this study, we found that LPS or poly(I:C)-induced TBK1 activity was significantly reduced in bone marrow-derived macrophage (BMDM) from OPTND477N/D477N mice. Consistent with this, the phosphorylation of IFN regulatory factor 3 (IRF3) and the production of IFN beta mRNA and secretion were reduced. Stimulation of BMDMs with LPS triggered the phosphorylation of OPTN, which was reversed by phosphatase treatment and prevented by pharmacological inhibition of both the canonical I kappa B kinases (IKK alpha/beta) and the IKK-related kinases (TBK1/IKK epsilon). In contrast, LPS-stimulated phosphorylation of OPTN(D477N) was markedly reduced in BMDMs from OPTND477N/D477N mice, and inhibition of the canonical IKKs alone prevented phosphorylation, providing further evidence that ubiquitin binding to OPTN contributes to LPS-induced TBK1 activation. TBK1 and IKK beta phosphorylated OPTN preferentially at Ser-177 and Ser-513, respectively, in vitro. In conclusion, our results suggest that OPTN binds to polyubiquity-lated species formed in response to LPS and poly(I:C), enhancing the activation of TBK1 that is required for optimal phosphorylation of IRF3 and production of IFN beta.

    AB - TANK-binding kinase (TBK1) is essential for transcription of the interferon (IFN) beta gene in response to lipopolysaccharide (LPS) and double-stranded RNA, but the molecular mechanisms that underlie the activation of TBK1 are incompletely understood. Previously, we identified the NF-kappa B essential modulator (NEMO)-related polyubiquitin-binding protein, optineurin (OPTN), as a novel binding partner of TBK1. To determine whether the ubiquitin-binding function of OPTN is involved in regulating TBK1 and IFN beta production, we generated a mouse in which wild-type optineurin was replaced by the polyubiquitin binding-defective mutant, OPTND477N/D477N. In this study, we found that LPS or poly(I:C)-induced TBK1 activity was significantly reduced in bone marrow-derived macrophage (BMDM) from OPTND477N/D477N mice. Consistent with this, the phosphorylation of IFN regulatory factor 3 (IRF3) and the production of IFN beta mRNA and secretion were reduced. Stimulation of BMDMs with LPS triggered the phosphorylation of OPTN, which was reversed by phosphatase treatment and prevented by pharmacological inhibition of both the canonical I kappa B kinases (IKK alpha/beta) and the IKK-related kinases (TBK1/IKK epsilon). In contrast, LPS-stimulated phosphorylation of OPTN(D477N) was markedly reduced in BMDMs from OPTND477N/D477N mice, and inhibition of the canonical IKKs alone prevented phosphorylation, providing further evidence that ubiquitin binding to OPTN contributes to LPS-induced TBK1 activation. TBK1 and IKK beta phosphorylated OPTN preferentially at Ser-177 and Ser-513, respectively, in vitro. In conclusion, our results suggest that OPTN binds to polyubiquity-lated species formed in response to LPS and poly(I:C), enhancing the activation of TBK1 that is required for optimal phosphorylation of IRF3 and production of IFN beta.

    KW - NF-KAPPA-B

    KW - SIGNALING PATHWAY

    KW - UBIQUITIN CHAINS

    KW - VIRAL-INFECTION

    KW - INNATE IMMUNITY

    KW - IFN RESPONSES

    KW - CUTTING EDGE

    KW - IKK-EPSILON

    KW - NEMO

    KW - DOMAIN

    U2 - 10.1074/jbc.M111.267567

    DO - 10.1074/jbc.M111.267567

    M3 - Article

    VL - 286

    SP - 35663

    EP - 35674

    JO - Journal of Biological Chemistry

    JF - Journal of Biological Chemistry

    SN - 0021-9258

    IS - 41

    ER -