Positive immunoselection--a method of isolating leukocytes from leukocytic reacted human cervical mucus samples

L. A. Thompson, M. J. Tomlinson, C. L. R. Barratt, A. E. Bolton, I. D. Cooke

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

In this brief communication we report a simple and accurate method of isolating and quantifying specific leukocytes from midcycle human cervical mucus, using monoclonal antibody-coated magnetic beads. Cervical mucus samples (pre- and postinsemination) were broken down enzymatically and incubated with a series of these beads. This method of positive immunoselection consistently retrieved representative levels of leukocytes (means = 73.8% +/- 1.59%; mean leukocyte retrieval rate +/- S.E.) from the cervical mucus samples. Significantly more leukocytes (P less than 0.0001) were isolated from the postinsemination samples, the predominant leukocyte of which was the neutrophil, which comprised 83% of the leukocyte population. These results reaffirm that a leukocytic influx is initiated across the human uterine cervix following the introduction of semen samples, the function of which is possibly phagocytic clearance of the nonfertilizing population of sperm.

Original languageEnglish
Pages (from-to)58-61
Number of pages4
JournalAmerican Journal of Reproductive Immunology
Volume26
Issue number2
DOIs
Publication statusPublished - Sep 1991

Keywords

  • Antibodies, Monoclonal
  • Cell Separation/methods
  • Cervix Mucus/cytology
  • Female
  • Humans
  • Insemination, Artificial, Heterologous
  • Leukocytes/immunology
  • Magnetics
  • Male
  • Spermatozoa/immunology

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