Positive immunoselection--a method of isolating leukocytes from leukocytic reacted human cervical mucus samples

TY - JOUR

T1 - Positive immunoselection--a method of isolating leukocytes from leukocytic reacted human cervical mucus samples

AU - Thompson, L. A.

AU - Tomlinson, M. J.

AU - Barratt, C. L. R.

AU - Bolton, A. E.

AU - Cooke, I. D.

PY - 1991/9

Y1 - 1991/9

N2 - In this brief communication we report a simple and accurate method of isolating and quantifying specific leukocytes from midcycle human cervical mucus, using monoclonal antibody-coated magnetic beads. Cervical mucus samples (pre- and postinsemination) were broken down enzymatically and incubated with a series of these beads. This method of positive immunoselection consistently retrieved representative levels of leukocytes (means = 73.8% +/- 1.59%; mean leukocyte retrieval rate +/- S.E.) from the cervical mucus samples. Significantly more leukocytes (P less than 0.0001) were isolated from the postinsemination samples, the predominant leukocyte of which was the neutrophil, which comprised 83% of the leukocyte population. These results reaffirm that a leukocytic influx is initiated across the human uterine cervix following the introduction of semen samples, the function of which is possibly phagocytic clearance of the nonfertilizing population of sperm.

AB - In this brief communication we report a simple and accurate method of isolating and quantifying specific leukocytes from midcycle human cervical mucus, using monoclonal antibody-coated magnetic beads. Cervical mucus samples (pre- and postinsemination) were broken down enzymatically and incubated with a series of these beads. This method of positive immunoselection consistently retrieved representative levels of leukocytes (means = 73.8% +/- 1.59%; mean leukocyte retrieval rate +/- S.E.) from the cervical mucus samples. Significantly more leukocytes (P less than 0.0001) were isolated from the postinsemination samples, the predominant leukocyte of which was the neutrophil, which comprised 83% of the leukocyte population. These results reaffirm that a leukocytic influx is initiated across the human uterine cervix following the introduction of semen samples, the function of which is possibly phagocytic clearance of the nonfertilizing population of sperm.

KW - Antibodies, Monoclonal

KW - Cell Separation/methods

KW - Cervix Mucus/cytology

KW - Female

KW - Humans

KW - Insemination, Artificial, Heterologous

KW - Leukocytes/immunology

KW - Magnetics

KW - Male

KW - Spermatozoa/immunology

U2 - 10.1111/j.1600-0897.1991.tb00971.x

DO - 10.1111/j.1600-0897.1991.tb00971.x

M3 - Article

C2 - 1768318

SN - 1046-7408

VL - 26

SP - 58

EP - 61

JO - American Journal of Reproductive Immunology

JF - American Journal of Reproductive Immunology

IS - 2

ER -