Positive selection for loss-of-function tat mutations identifies critical residues required for TatA activity

Matthew G. Hicks, Philip A. Lee, George Georgiou, Ben C. Berks, Tracy Palmer

    Research output: Contribution to journalArticlepeer-review

    31 Citations (Scopus)

    Abstract

    The Tat system, found in the cytoplasmic membrane of many bacteria, is a general export pathway for folded proteins. Here we describe the development of a method, based on the transport of chloramphenicol acetyltransferase, that allows positive selection of mutants defective in Tat function. We have demonstrated the utility of this method by selecting novel loss-of-function alleles of tatA from a pool of random taA mutations. Most of the mutations that were isolated fall in the amphipathic region of TatA, emphasizing the pivotal role that this part of the protein plays in TatA function.

    Original languageEnglish
    Pages (from-to)2920-2925
    Number of pages6
    JournalJournal of Bacteriology
    Volume187
    Issue number8
    DOIs
    Publication statusPublished - 2005

    Cite this