Possibilities for human skin characterization based on strongly reduced Raman spectroscopic information

A.C. Baclig, T.C. Bakker Schut, G.M. O'Regan, A.D. Irvine, W.H.I. McLean, G.J. Puppels, P.J. Caspers

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    Loss-of-function mutations in the gene coding for filaggrin are the single most important risk factor for development of atopic dermatitis and associated allergic rhinitis and asthma. Filaggrin is enzymatically degraded to natural moisturizing factor (NMF) in the stratum corneum (SC). In vivo Raman spectra of human skin can be used to quantify the NMF concentration in SC and thereby identify carriers of a loss-of-function mutation in the gene coding for filaggrin, which results in decreased NMF content. Here, we demonstrate that strongly reduced Raman spectral information is sufficient to make this differentiation. This is an important step towards development of a dedicated diagnostic device of reduced complexity, size and cost as compared to current state-of-the-art Raman equipment. A genetic algorithm was used to select the spectral regions needed to classify skin based on normal or reduced NMF content in SC. Using the NMF content based on full spectral information as gold standard, only four Raman regions were required to create a linear discriminant analysis model that can differentiate between skin with low NMF and skin with normal NMF with a prediction accuracy of 93 %.
    Original languageEnglish
    Pages (from-to)340-345
    Number of pages6
    JournalJournal of Raman Spectroscopy
    Volume44
    Issue number3
    DOIs
    Publication statusPublished - 2013

    Fingerprint

    Skin
    Genes
    Discriminant analysis
    Raman scattering
    Genetic algorithms
    filaggrin
    Costs

    Cite this

    Baclig, A. C., Bakker Schut, T. C., O'Regan, G. M., Irvine, A. D., McLean, W. H. I., Puppels, G. J., & Caspers, P. J. (2013). Possibilities for human skin characterization based on strongly reduced Raman spectroscopic information. Journal of Raman Spectroscopy, 44(3), 340-345. https://doi.org/10.1002/jrs.4198
    Baclig, A.C. ; Bakker Schut, T.C. ; O'Regan, G.M. ; Irvine, A.D. ; McLean, W.H.I. ; Puppels, G.J. ; Caspers, P.J. / Possibilities for human skin characterization based on strongly reduced Raman spectroscopic information. In: Journal of Raman Spectroscopy. 2013 ; Vol. 44, No. 3. pp. 340-345.
    @article{d713c17c63ef4d22bfa77c26d46ae672,
    title = "Possibilities for human skin characterization based on strongly reduced Raman spectroscopic information",
    abstract = "Loss-of-function mutations in the gene coding for filaggrin are the single most important risk factor for development of atopic dermatitis and associated allergic rhinitis and asthma. Filaggrin is enzymatically degraded to natural moisturizing factor (NMF) in the stratum corneum (SC). In vivo Raman spectra of human skin can be used to quantify the NMF concentration in SC and thereby identify carriers of a loss-of-function mutation in the gene coding for filaggrin, which results in decreased NMF content. Here, we demonstrate that strongly reduced Raman spectral information is sufficient to make this differentiation. This is an important step towards development of a dedicated diagnostic device of reduced complexity, size and cost as compared to current state-of-the-art Raman equipment. A genetic algorithm was used to select the spectral regions needed to classify skin based on normal or reduced NMF content in SC. Using the NMF content based on full spectral information as gold standard, only four Raman regions were required to create a linear discriminant analysis model that can differentiate between skin with low NMF and skin with normal NMF with a prediction accuracy of 93 {\%}.",
    author = "A.C. Baclig and {Bakker Schut}, T.C. and G.M. O'Regan and A.D. Irvine and W.H.I. McLean and G.J. Puppels and P.J. Caspers",
    note = "Copyright 2013 Elsevier B.V., All rights reserved.",
    year = "2013",
    doi = "10.1002/jrs.4198",
    language = "English",
    volume = "44",
    pages = "340--345",
    journal = "Journal of Raman Spectroscopy",
    issn = "0377-0486",
    publisher = "Wiley",
    number = "3",

    }

    Baclig, AC, Bakker Schut, TC, O'Regan, GM, Irvine, AD, McLean, WHI, Puppels, GJ & Caspers, PJ 2013, 'Possibilities for human skin characterization based on strongly reduced Raman spectroscopic information', Journal of Raman Spectroscopy, vol. 44, no. 3, pp. 340-345. https://doi.org/10.1002/jrs.4198

    Possibilities for human skin characterization based on strongly reduced Raman spectroscopic information. / Baclig, A.C.; Bakker Schut, T.C.; O'Regan, G.M.; Irvine, A.D.; McLean, W.H.I.; Puppels, G.J.; Caspers, P.J.

    In: Journal of Raman Spectroscopy, Vol. 44, No. 3, 2013, p. 340-345.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Possibilities for human skin characterization based on strongly reduced Raman spectroscopic information

    AU - Baclig, A.C.

    AU - Bakker Schut, T.C.

    AU - O'Regan, G.M.

    AU - Irvine, A.D.

    AU - McLean, W.H.I.

    AU - Puppels, G.J.

    AU - Caspers, P.J.

    N1 - Copyright 2013 Elsevier B.V., All rights reserved.

    PY - 2013

    Y1 - 2013

    N2 - Loss-of-function mutations in the gene coding for filaggrin are the single most important risk factor for development of atopic dermatitis and associated allergic rhinitis and asthma. Filaggrin is enzymatically degraded to natural moisturizing factor (NMF) in the stratum corneum (SC). In vivo Raman spectra of human skin can be used to quantify the NMF concentration in SC and thereby identify carriers of a loss-of-function mutation in the gene coding for filaggrin, which results in decreased NMF content. Here, we demonstrate that strongly reduced Raman spectral information is sufficient to make this differentiation. This is an important step towards development of a dedicated diagnostic device of reduced complexity, size and cost as compared to current state-of-the-art Raman equipment. A genetic algorithm was used to select the spectral regions needed to classify skin based on normal or reduced NMF content in SC. Using the NMF content based on full spectral information as gold standard, only four Raman regions were required to create a linear discriminant analysis model that can differentiate between skin with low NMF and skin with normal NMF with a prediction accuracy of 93 %.

    AB - Loss-of-function mutations in the gene coding for filaggrin are the single most important risk factor for development of atopic dermatitis and associated allergic rhinitis and asthma. Filaggrin is enzymatically degraded to natural moisturizing factor (NMF) in the stratum corneum (SC). In vivo Raman spectra of human skin can be used to quantify the NMF concentration in SC and thereby identify carriers of a loss-of-function mutation in the gene coding for filaggrin, which results in decreased NMF content. Here, we demonstrate that strongly reduced Raman spectral information is sufficient to make this differentiation. This is an important step towards development of a dedicated diagnostic device of reduced complexity, size and cost as compared to current state-of-the-art Raman equipment. A genetic algorithm was used to select the spectral regions needed to classify skin based on normal or reduced NMF content in SC. Using the NMF content based on full spectral information as gold standard, only four Raman regions were required to create a linear discriminant analysis model that can differentiate between skin with low NMF and skin with normal NMF with a prediction accuracy of 93 %.

    UR - http://www.scopus.com/inward/record.url?scp=84875699125&partnerID=8YFLogxK

    U2 - 10.1002/jrs.4198

    DO - 10.1002/jrs.4198

    M3 - Article

    VL - 44

    SP - 340

    EP - 345

    JO - Journal of Raman Spectroscopy

    JF - Journal of Raman Spectroscopy

    SN - 0377-0486

    IS - 3

    ER -