Potato late blight field resistance from QTL dPI09c is conferred by the NB-LRR gene R8

Rui Jiang; Jingcai Li; Zhendong Tian; Juan Du; Miles Armstrong; Katie Baker; Joanne Tze-Yin Lim; Jack H. Vossen; Huan He; Leticia Portal; Jun Zhou; Merideth Bonierbale; Ingo Hein; Hannele Lindqvist-Kreuze; Conghua Xie

doi:10.1093/jxb/ery021

Potato late blight field resistance from QTL dPI09c is conferred by the NB-LRR gene R8

Rui Jiang, Jingcai Li, Zhendong Tian, Juan Du, Miles Armstrong, Katie Baker, Joanne Tze-Yin Lim, Jack H. Vossen, Huan He, Leticia Portal, Jun Zhou, Merideth Bonierbale, Ingo Hein (Lead / Corresponding author), Hannele Lindqvist-Kreuze (Lead / Corresponding author), Conghua Xie (Lead / Corresponding author)

Plant Sciences

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Abstract

Following the often short-lived protection that major nucleotide binding, leucine-rich-repeat (NB-LRR) resistance genes offer against the potato pathogen Phytophthora infestans, field resistance was thought to provide a more durable alternative to prevent late blight disease. We previously identified the QTL dPI09c on potato chromosome 9 as a more durable field resistance source against late blight. Here, the resistance QTL was fine-mapped to a 186 kb region. The interval corresponds to a larger, 389 kb, genomic region in the potato reference genome of Solanum tuberosum Group Phureja doubled monoploid clone DM1-3 (DM) and from which functional NB-LRRs R8, R9a, Rpi-moc1, and Rpi-vnt1 have arisen independently in wild species. dRenSeq analysis of parental clones alongside resistant and susceptible bulks of the segregating population B3C1HP showed full sequence representation of R8. This was independently validated using long-range PCR and screening of a bespoke bacterial artificial chromosome library. The latter enabled a comparative analysis of the sequence variation in this locus in diverse Solanaceae. We reveal for the first time that broad spectrum and durable field resistance against P. infestans is conferred by the NB-LRR gene R8, which is thought to provide narrow spectrum race-specific resistance.

Original language	English
Pages (from-to)	1545-1555
Number of pages	11
Journal	Journal of Experimental Botany
Volume	69
Issue number	7
Early online date	27 Jan 2018
DOIs	https://doi.org/10.1093/jxb/ery021
Publication status	Published - 16 Mar 2018

Keywords

R gene
dRenSeq
field resistance
late blight
map-based cloning
potato

ASJC Scopus subject areas

Physiology
Plant Science

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10.1093/jxb/ery021Licence: CC BY

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© The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
Final published version, 1.4 MBLicence: CC BY

Cite this

@article{e23e30fb21874ebd91e9ecab73c89162,

title = "Potato late blight field resistance from QTL dPI09c is conferred by the NB-LRR gene R8",

abstract = "Following the often short-lived protection that major nucleotide binding, leucine-rich-repeat (NB-LRR) resistance genes offer against the potato pathogen Phytophthora infestans, field resistance was thought to provide a more durable alternative to prevent late blight disease. We previously identified the QTL dPI09c on potato chromosome 9 as a more durable field resistance source against late blight. Here, the resistance QTL was fine-mapped to a 186 kb region. The interval corresponds to a larger, 389 kb, genomic region in the potato reference genome of Solanum tuberosum Group Phureja doubled monoploid clone DM1-3 (DM) and from which functional NB-LRRs R8, R9a, Rpi-moc1, and Rpi-vnt1 have arisen independently in wild species. dRenSeq analysis of parental clones alongside resistant and susceptible bulks of the segregating population B3C1HP showed full sequence representation of R8. This was independently validated using long-range PCR and screening of a bespoke bacterial artificial chromosome library. The latter enabled a comparative analysis of the sequence variation in this locus in diverse Solanaceae. We reveal for the first time that broad spectrum and durable field resistance against P. infestans is conferred by the NB-LRR gene R8, which is thought to provide narrow spectrum race-specific resistance.",

keywords = "R gene, dRenSeq, field resistance, late blight, map-based cloning, potato",

author = "Rui Jiang and Jingcai Li and Zhendong Tian and Juan Du and Miles Armstrong and Katie Baker and Lim, {Joanne Tze-Yin} and Vossen, {Jack H.} and Huan He and Leticia Portal and Jun Zhou and Merideth Bonierbale and Ingo Hein and Hannele Lindqvist-Kreuze and Conghua Xie",

note = "We appreciated Miss Lu Li, Mr. Caihua Zhu, Mr. Gege Li for helping with genotyping. We thank Mr. Jiadong Li for assisting in long-range PCR. Acknowledgement to the funds from the National Natural Science Foundation of China to C. Xie (31261140362), the CGIAR Research Program on RTB (2012 to 2015) and USAID (2015-2017) to H. Lindqvist-Kreuze.",

year = "2018",

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doi = "10.1093/jxb/ery021",

language = "English",

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T1 - Potato late blight field resistance from QTL dPI09c is conferred by the NB-LRR gene R8

AU - Jiang, Rui

AU - Li, Jingcai

AU - Tian, Zhendong

AU - Du, Juan

AU - Armstrong, Miles

AU - Baker, Katie

AU - Lim, Joanne Tze-Yin

AU - Vossen, Jack H.

AU - He, Huan

AU - Portal, Leticia

AU - Zhou, Jun

AU - Bonierbale, Merideth

AU - Hein, Ingo

AU - Lindqvist-Kreuze, Hannele

AU - Xie, Conghua

N1 - We appreciated Miss Lu Li, Mr. Caihua Zhu, Mr. Gege Li for helping with genotyping. We thank Mr. Jiadong Li for assisting in long-range PCR. Acknowledgement to the funds from the National Natural Science Foundation of China to C. Xie (31261140362), the CGIAR Research Program on RTB (2012 to 2015) and USAID (2015-2017) to H. Lindqvist-Kreuze.

PY - 2018/3/16

Y1 - 2018/3/16

N2 - Following the often short-lived protection that major nucleotide binding, leucine-rich-repeat (NB-LRR) resistance genes offer against the potato pathogen Phytophthora infestans, field resistance was thought to provide a more durable alternative to prevent late blight disease. We previously identified the QTL dPI09c on potato chromosome 9 as a more durable field resistance source against late blight. Here, the resistance QTL was fine-mapped to a 186 kb region. The interval corresponds to a larger, 389 kb, genomic region in the potato reference genome of Solanum tuberosum Group Phureja doubled monoploid clone DM1-3 (DM) and from which functional NB-LRRs R8, R9a, Rpi-moc1, and Rpi-vnt1 have arisen independently in wild species. dRenSeq analysis of parental clones alongside resistant and susceptible bulks of the segregating population B3C1HP showed full sequence representation of R8. This was independently validated using long-range PCR and screening of a bespoke bacterial artificial chromosome library. The latter enabled a comparative analysis of the sequence variation in this locus in diverse Solanaceae. We reveal for the first time that broad spectrum and durable field resistance against P. infestans is conferred by the NB-LRR gene R8, which is thought to provide narrow spectrum race-specific resistance.

AB - Following the often short-lived protection that major nucleotide binding, leucine-rich-repeat (NB-LRR) resistance genes offer against the potato pathogen Phytophthora infestans, field resistance was thought to provide a more durable alternative to prevent late blight disease. We previously identified the QTL dPI09c on potato chromosome 9 as a more durable field resistance source against late blight. Here, the resistance QTL was fine-mapped to a 186 kb region. The interval corresponds to a larger, 389 kb, genomic region in the potato reference genome of Solanum tuberosum Group Phureja doubled monoploid clone DM1-3 (DM) and from which functional NB-LRRs R8, R9a, Rpi-moc1, and Rpi-vnt1 have arisen independently in wild species. dRenSeq analysis of parental clones alongside resistant and susceptible bulks of the segregating population B3C1HP showed full sequence representation of R8. This was independently validated using long-range PCR and screening of a bespoke bacterial artificial chromosome library. The latter enabled a comparative analysis of the sequence variation in this locus in diverse Solanaceae. We reveal for the first time that broad spectrum and durable field resistance against P. infestans is conferred by the NB-LRR gene R8, which is thought to provide narrow spectrum race-specific resistance.

KW - R gene

KW - dRenSeq

KW - field resistance

KW - late blight

KW - map-based cloning

KW - potato

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U2 - 10.1093/jxb/ery021

DO - 10.1093/jxb/ery021

M3 - Article

C2 - 29385612

SN - 0022-0957

VL - 69

SP - 1545

EP - 1555

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

IS - 7

ER -

Potato late blight field resistance from QTL dPI09c is conferred by the NB-LRR gene R8

Abstract

Keywords

ASJC Scopus subject areas

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