Potato late blight field resistance from QTL dPI09c is conferred by the NB-LRR gene R8

Rui Jiang, Jingcai Li, Zhendong Tian, Juan Du, Miles Armstrong, Katie Baker, Joanne Tze-Yin Lim, Jack H. Vossen, Huan He, Leticia Portal, Jun Zhou, Merideth Bonierbale, Ingo Hein (Lead / Corresponding author), Hannele Lindqvist-Kreuze (Lead / Corresponding author), Conghua Xie (Lead / Corresponding author)

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Abstract

Following the often short-lived protection that major nucleotide binding, leucine-rich-repeat (NB-LRR) resistance genes offer against the potato pathogen Phytophthora infestans, field resistance was thought to provide a more durable alternative to prevent late blight disease. We previously identified the QTL dPI09c on potato chromosome 9 as a more durable field resistance source against late blight. Here, the resistance QTL was fine-mapped to a 186 kb region. The interval corresponds to a larger, 389 kb, genomic region in the potato reference genome of Solanum tuberosum Group Phureja doubled monoploid clone DM1-3 (DM) and from which functional NB-LRRs R8, R9a, Rpi-moc1, and Rpi-vnt1 have arisen independently in wild species. dRenSeq analysis of parental clones alongside resistant and susceptible bulks of the segregating population B3C1HP showed full sequence representation of R8. This was independently validated using long-range PCR and screening of a bespoke bacterial artificial chromosome library. The latter enabled a comparative analysis of the sequence variation in this locus in diverse Solanaceae. We reveal for the first time that broad spectrum and durable field resistance against P. infestans is conferred by the NB-LRR gene R8, which is thought to provide narrow spectrum race-specific resistance.

Original languageEnglish
Pages (from-to)1545-1555
Number of pages11
JournalJournal of Experimental Botany
Volume69
Issue number7
Early online date27 Jan 2018
DOIs
Publication statusPublished - 16 Mar 2018

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Keywords

  • R gene
  • dRenSeq
  • field resistance
  • late blight
  • map-based cloning
  • potato

Cite this

Jiang, R., Li, J., Tian, Z., Du, J., Armstrong, M., Baker, K., Lim, J. T-Y., Vossen, J. H., He, H., Portal, L., Zhou, J., Bonierbale, M., Hein, I., Lindqvist-Kreuze, H., & Xie, C. (2018). Potato late blight field resistance from QTL dPI09c is conferred by the NB-LRR gene R8. Journal of Experimental Botany, 69(7), 1545-1555. https://doi.org/10.1093/jxb/ery021