Potato (Solanum tuberosum) invertase-encoding cDNAs and their differential expression

Peter E. Hedley; Gordon C. Machray; Howard V. Davies; Lindsay Burch; Robbie Waugh

doi:10.1016/0378-1119(94)90007-8

Potato (Solanum tuberosum) invertase-encoding cDNAs and their differential expression

Peter E. Hedley, Gordon C. Machray (Lead / Corresponding author), Howard V. Davies, Lindsay Burch, Robbie Waugh

Plant Sciences

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

A full-length cDNA clone encoding a potato invertase (Inv) has been isolated. It is highly related (77% nucleotide identity) to a previously characterised potato cDNA clone encoding a putative extracellular Inv. These Inv genes encode a subfamily of apoplastic enzymes which are shown to be distinct, on the basis of sequence similarity, from the related subfamily of vacuolar enzymes. In order to differentiate between the expression of the two potato genes encoding apoplastic Inv, a single-stranded conformational polymorphism (SSCP) assay was developed for products generated by reverse transcription-polymerase chain reaction (RT-PCR) utilising primers designed to amplify both potato sequences. Using this approach, we have shown that these two identified Inv from potato are expressed in a tissue-specific and developmentally regulated manner.

Original language	English
Pages (from-to)	211-214
Number of pages	4
Journal	Gene
Volume	145
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(94)90007-8
Publication status	Published - 5 Aug 1994

Keywords

apoplastic enzyme
reverse transcription-polymerase chain reaction
single-stranded conformational polymorphism
β-Fructosidase

ASJC Scopus subject areas

Medicine(all)
Genetics

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10.1016/0378-1119(94)90007-8

Cite this

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title = "Potato (Solanum tuberosum) invertase-encoding cDNAs and their differential expression",

abstract = "A full-length cDNA clone encoding a potato invertase (Inv) has been isolated. It is highly related (77% nucleotide identity) to a previously characterised potato cDNA clone encoding a putative extracellular Inv. These Inv genes encode a subfamily of apoplastic enzymes which are shown to be distinct, on the basis of sequence similarity, from the related subfamily of vacuolar enzymes. In order to differentiate between the expression of the two potato genes encoding apoplastic Inv, a single-stranded conformational polymorphism (SSCP) assay was developed for products generated by reverse transcription-polymerase chain reaction (RT-PCR) utilising primers designed to amplify both potato sequences. Using this approach, we have shown that these two identified Inv from potato are expressed in a tissue-specific and developmentally regulated manner.",

keywords = "apoplastic enzyme, reverse transcription-polymerase chain reaction, single-stranded conformational polymorphism, β-Fructosidase",

author = "Hedley, {Peter E.} and Machray, {Gordon C.} and Davies, {Howard V.} and Lindsay Burch and Robbie Waugh",

year = "1994",

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T1 - Potato (Solanum tuberosum) invertase-encoding cDNAs and their differential expression

AU - Hedley, Peter E.

AU - Machray, Gordon C.

AU - Davies, Howard V.

AU - Burch, Lindsay

AU - Waugh, Robbie

PY - 1994/8/5

Y1 - 1994/8/5

N2 - A full-length cDNA clone encoding a potato invertase (Inv) has been isolated. It is highly related (77% nucleotide identity) to a previously characterised potato cDNA clone encoding a putative extracellular Inv. These Inv genes encode a subfamily of apoplastic enzymes which are shown to be distinct, on the basis of sequence similarity, from the related subfamily of vacuolar enzymes. In order to differentiate between the expression of the two potato genes encoding apoplastic Inv, a single-stranded conformational polymorphism (SSCP) assay was developed for products generated by reverse transcription-polymerase chain reaction (RT-PCR) utilising primers designed to amplify both potato sequences. Using this approach, we have shown that these two identified Inv from potato are expressed in a tissue-specific and developmentally regulated manner.

AB - A full-length cDNA clone encoding a potato invertase (Inv) has been isolated. It is highly related (77% nucleotide identity) to a previously characterised potato cDNA clone encoding a putative extracellular Inv. These Inv genes encode a subfamily of apoplastic enzymes which are shown to be distinct, on the basis of sequence similarity, from the related subfamily of vacuolar enzymes. In order to differentiate between the expression of the two potato genes encoding apoplastic Inv, a single-stranded conformational polymorphism (SSCP) assay was developed for products generated by reverse transcription-polymerase chain reaction (RT-PCR) utilising primers designed to amplify both potato sequences. Using this approach, we have shown that these two identified Inv from potato are expressed in a tissue-specific and developmentally regulated manner.

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KW - reverse transcription-polymerase chain reaction

KW - single-stranded conformational polymorphism

KW - β-Fructosidase

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Potato (Solanum tuberosum) invertase-encoding cDNAs and their differential expression

Abstract

Keywords

ASJC Scopus subject areas

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