Potato (Solanum tuberosum) invertase-encoding cDNAs and their differential expression

Peter E. Hedley, Gordon C. Machray (Lead / Corresponding author), Howard V. Davies, Lindsay Burch, Robbie Waugh

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A full-length cDNA clone encoding a potato invertase (Inv) has been isolated. It is highly related (77% nucleotide identity) to a previously characterised potato cDNA clone encoding a putative extracellular Inv. These Inv genes encode a subfamily of apoplastic enzymes which are shown to be distinct, on the basis of sequence similarity, from the related subfamily of vacuolar enzymes. In order to differentiate between the expression of the two potato genes encoding apoplastic Inv, a single-stranded conformational polymorphism (SSCP) assay was developed for products generated by reverse transcription-polymerase chain reaction (RT-PCR) utilising primers designed to amplify both potato sequences. Using this approach, we have shown that these two identified Inv from potato are expressed in a tissue-specific and developmentally regulated manner.

Original languageEnglish
Pages (from-to)211-214
Number of pages4
JournalGene
Volume145
Issue number2
DOIs
Publication statusPublished - 5 Aug 1994

Fingerprint

beta-Fructofuranosidase
Solanum tuberosum
Complementary DNA
Clone Cells
Single-Stranded Conformational Polymorphism
Enzymes
Genes
Reverse Transcription
Nucleotides
Polymerase Chain Reaction

Keywords

  • apoplastic enzyme
  • reverse transcription-polymerase chain reaction
  • single-stranded conformational polymorphism
  • β-Fructosidase

Cite this

Hedley, Peter E. ; Machray, Gordon C. ; Davies, Howard V. ; Burch, Lindsay ; Waugh, Robbie. / Potato (Solanum tuberosum) invertase-encoding cDNAs and their differential expression. In: Gene. 1994 ; Vol. 145, No. 2. pp. 211-214.
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Potato (Solanum tuberosum) invertase-encoding cDNAs and their differential expression. / Hedley, Peter E.; Machray, Gordon C. (Lead / Corresponding author); Davies, Howard V.; Burch, Lindsay; Waugh, Robbie.

In: Gene, Vol. 145, No. 2, 05.08.1994, p. 211-214.

Research output: Contribution to journalArticle

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