Potato virus X-induced gene silencing in leaves and tubers of potato

Odile Faivre-Rampant, Eleanor M Gilroy, Katarina Hrubikova, Ingo Hein, Steve Millam, Gary J Loake, Paul Birch, Mark Taylor, Christophe Lacomme (Lead / Corresponding author)

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

Virus induced gene silencing (VIGS) is increasingly used to generate transient loss-of-function assays and has potential as a powerful reverse-genetics tool in functional genomic programs as a more rapid alternative to stable transformation. A previously described potato virus X (PVX) VIGS vector has been shown to trigger silencing in the permissive host Nicotiana benthamiana. This paper demonstrates that a PVX-based VIGS vector is also effective in triggering a VIGS response in both diploid and cultivated tetraploid Solanum species. We show that systemic silencing of a phytoene desaturase gene is observed and maintained throughout the foliar tissues of potato plants and was also observed in tubers. Here we report that VIGS can be triggered and sustained on in vitro micropropagated tetraploid potato for several cycles and on in vitro generated microtubers. This approach will facilitate large-scale functional analysis of potato expressed sequence tags and provide a noninvasive reverse-genetic approach to study mechanisms involved in tuber and microtuber development.

Original languageEnglish
Pages (from-to)1308-1316
Number of pages9
JournalPlant Physiology
Volume134
Issue number4
DOIs
Publication statusPublished - Apr 2004

Fingerprint

Potexvirus
Potato virus X
Gene Silencing
gene silencing
Solanum tuberosum
tubers
potatoes
Viruses
viruses
Reverse Genetics
Tetraploidy
leaves
tetraploidy
Solanum
Nicotiana benthamiana
Expressed Sequence Tags
expressed sequence tags
Diploidy
Tobacco
diploidy

Keywords

  • Base Sequence
  • Culture Techniques
  • Diploidy
  • Gene Silencing/physiology
  • Genetic Vectors/genetics
  • Molecular Sequence Data
  • Oxidoreductases/genetics
  • Plant Leaves/genetics
  • Plant Tubers/genetics
  • Polyploidy
  • Potexvirus/genetics
  • Sequence Homology, Nucleic Acid
  • Solanum tuberosum/genetics

Cite this

Faivre-Rampant, O., Gilroy, E. M., Hrubikova, K., Hein, I., Millam, S., Loake, G. J., ... Lacomme, C. (2004). Potato virus X-induced gene silencing in leaves and tubers of potato. Plant Physiology, 134(4), 1308-1316. https://doi.org/10.1104/pp.103.037507
Faivre-Rampant, Odile ; Gilroy, Eleanor M ; Hrubikova, Katarina ; Hein, Ingo ; Millam, Steve ; Loake, Gary J ; Birch, Paul ; Taylor, Mark ; Lacomme, Christophe. / Potato virus X-induced gene silencing in leaves and tubers of potato. In: Plant Physiology. 2004 ; Vol. 134, No. 4. pp. 1308-1316.
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abstract = "Virus induced gene silencing (VIGS) is increasingly used to generate transient loss-of-function assays and has potential as a powerful reverse-genetics tool in functional genomic programs as a more rapid alternative to stable transformation. A previously described potato virus X (PVX) VIGS vector has been shown to trigger silencing in the permissive host Nicotiana benthamiana. This paper demonstrates that a PVX-based VIGS vector is also effective in triggering a VIGS response in both diploid and cultivated tetraploid Solanum species. We show that systemic silencing of a phytoene desaturase gene is observed and maintained throughout the foliar tissues of potato plants and was also observed in tubers. Here we report that VIGS can be triggered and sustained on in vitro micropropagated tetraploid potato for several cycles and on in vitro generated microtubers. This approach will facilitate large-scale functional analysis of potato expressed sequence tags and provide a noninvasive reverse-genetic approach to study mechanisms involved in tuber and microtuber development.",
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Faivre-Rampant, O, Gilroy, EM, Hrubikova, K, Hein, I, Millam, S, Loake, GJ, Birch, P, Taylor, M & Lacomme, C 2004, 'Potato virus X-induced gene silencing in leaves and tubers of potato', Plant Physiology, vol. 134, no. 4, pp. 1308-1316. https://doi.org/10.1104/pp.103.037507

Potato virus X-induced gene silencing in leaves and tubers of potato. / Faivre-Rampant, Odile; Gilroy, Eleanor M; Hrubikova, Katarina; Hein, Ingo; Millam, Steve; Loake, Gary J; Birch, Paul; Taylor, Mark; Lacomme, Christophe (Lead / Corresponding author).

In: Plant Physiology, Vol. 134, No. 4, 04.2004, p. 1308-1316.

Research output: Contribution to journalArticle

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T1 - Potato virus X-induced gene silencing in leaves and tubers of potato

AU - Faivre-Rampant, Odile

AU - Gilroy, Eleanor M

AU - Hrubikova, Katarina

AU - Hein, Ingo

AU - Millam, Steve

AU - Loake, Gary J

AU - Birch, Paul

AU - Taylor, Mark

AU - Lacomme, Christophe

PY - 2004/4

Y1 - 2004/4

N2 - Virus induced gene silencing (VIGS) is increasingly used to generate transient loss-of-function assays and has potential as a powerful reverse-genetics tool in functional genomic programs as a more rapid alternative to stable transformation. A previously described potato virus X (PVX) VIGS vector has been shown to trigger silencing in the permissive host Nicotiana benthamiana. This paper demonstrates that a PVX-based VIGS vector is also effective in triggering a VIGS response in both diploid and cultivated tetraploid Solanum species. We show that systemic silencing of a phytoene desaturase gene is observed and maintained throughout the foliar tissues of potato plants and was also observed in tubers. Here we report that VIGS can be triggered and sustained on in vitro micropropagated tetraploid potato for several cycles and on in vitro generated microtubers. This approach will facilitate large-scale functional analysis of potato expressed sequence tags and provide a noninvasive reverse-genetic approach to study mechanisms involved in tuber and microtuber development.

AB - Virus induced gene silencing (VIGS) is increasingly used to generate transient loss-of-function assays and has potential as a powerful reverse-genetics tool in functional genomic programs as a more rapid alternative to stable transformation. A previously described potato virus X (PVX) VIGS vector has been shown to trigger silencing in the permissive host Nicotiana benthamiana. This paper demonstrates that a PVX-based VIGS vector is also effective in triggering a VIGS response in both diploid and cultivated tetraploid Solanum species. We show that systemic silencing of a phytoene desaturase gene is observed and maintained throughout the foliar tissues of potato plants and was also observed in tubers. Here we report that VIGS can be triggered and sustained on in vitro micropropagated tetraploid potato for several cycles and on in vitro generated microtubers. This approach will facilitate large-scale functional analysis of potato expressed sequence tags and provide a noninvasive reverse-genetic approach to study mechanisms involved in tuber and microtuber development.

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KW - Culture Techniques

KW - Diploidy

KW - Gene Silencing/physiology

KW - Genetic Vectors/genetics

KW - Molecular Sequence Data

KW - Oxidoreductases/genetics

KW - Plant Leaves/genetics

KW - Plant Tubers/genetics

KW - Polyploidy

KW - Potexvirus/genetics

KW - Sequence Homology, Nucleic Acid

KW - Solanum tuberosum/genetics

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JF - Plant Physiology

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ER -

Faivre-Rampant O, Gilroy EM, Hrubikova K, Hein I, Millam S, Loake GJ et al. Potato virus X-induced gene silencing in leaves and tubers of potato. Plant Physiology. 2004 Apr;134(4):1308-1316. https://doi.org/10.1104/pp.103.037507