POU2F1 activity regulates HOXD10 and HOXD11 promoting a proliferative and invasive phenotype in Head and Neck cancer

Daniel J. Sharpe, Katy S. Orr, Michael Moran, Sharon J. White, Stephen McQuaid, Terence R. Lappin, Alexander Thompson, Jacqueline A. James (Lead / Corresponding author)

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    Abstract

    HOX genes are master regulators of organ morphogenesis and cell differentiation during embryonic development, and continue to be expressed throughout post-natal life. To test the hypothesis that HOX genes are dysregulated in head and neck squamous cell carcinoma (HNSCC) we defined their expression profile, and investigated the function, transcriptional regulation and clinical relevance of a subset of highly expressed HOXD genes. Two HOXD genes, D10 and D11, showed strikingly high levels in HNSCC cell lines, patient tumor samples and publicly available datasets. Knockdown of HOXD10 in HNSCC cells caused decreased proliferation and invasion, whereas knockdown of HOXD11 reduced only invasion. POU2F1 consensus sequences were identified in the 5' DNA of HOXD10 and D11. Knockdown of POU2F1 significantly reduced expression of HOXD10 and D11 and inhibited HNSCC proliferation. Luciferase reporter constructs of the HOXD10 and D11 promoters confirmed that POU2F1 consensus binding sites are required for optimal promoter activity. Utilizing patient tumor samples a significant association was found between immunohistochemical staining of HOXD10 and both the overall and the disease-specific survival, adding further support that HOXD10 is dysregulated in head and neck cancer. Additional studies are now warranted to fully evaluate HOXD10 as a prognostic tool in head and neck cancers.
    Original languageEnglish
    Pages (from-to)8803-8815
    Number of pages13
    JournalOncotarget
    Volume5
    Issue number18
    DOIs
    Publication statusPublished - 16 Sep 2014

    Fingerprint

    Head and Neck Neoplasms
    Phenotype
    Genes
    Consensus Sequence
    Regulator Genes
    Tumor Cell Line
    Luciferases
    Morphogenesis
    Embryonic Development
    Cell Differentiation
    Binding Sites
    Cell Proliferation
    Staining and Labeling
    Survival
    Carcinoma, squamous cell of head and neck
    DNA
    Neoplasms

    Cite this

    Sharpe, Daniel J. ; Orr, Katy S. ; Moran, Michael ; White, Sharon J. ; McQuaid, Stephen ; Lappin, Terence R. ; Thompson, Alexander ; James, Jacqueline A. / POU2F1 activity regulates HOXD10 and HOXD11 promoting a proliferative and invasive phenotype in Head and Neck cancer. In: Oncotarget. 2014 ; Vol. 5, No. 18. pp. 8803-8815.
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    abstract = "HOX genes are master regulators of organ morphogenesis and cell differentiation during embryonic development, and continue to be expressed throughout post-natal life. To test the hypothesis that HOX genes are dysregulated in head and neck squamous cell carcinoma (HNSCC) we defined their expression profile, and investigated the function, transcriptional regulation and clinical relevance of a subset of highly expressed HOXD genes. Two HOXD genes, D10 and D11, showed strikingly high levels in HNSCC cell lines, patient tumor samples and publicly available datasets. Knockdown of HOXD10 in HNSCC cells caused decreased proliferation and invasion, whereas knockdown of HOXD11 reduced only invasion. POU2F1 consensus sequences were identified in the 5' DNA of HOXD10 and D11. Knockdown of POU2F1 significantly reduced expression of HOXD10 and D11 and inhibited HNSCC proliferation. Luciferase reporter constructs of the HOXD10 and D11 promoters confirmed that POU2F1 consensus binding sites are required for optimal promoter activity. Utilizing patient tumor samples a significant association was found between immunohistochemical staining of HOXD10 and both the overall and the disease-specific survival, adding further support that HOXD10 is dysregulated in head and neck cancer. Additional studies are now warranted to fully evaluate HOXD10 as a prognostic tool in head and neck cancers.",
    author = "Sharpe, {Daniel J.} and Orr, {Katy S.} and Michael Moran and White, {Sharon J.} and Stephen McQuaid and Lappin, {Terence R.} and Alexander Thompson and James, {Jacqueline A.}",
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    Sharpe, DJ, Orr, KS, Moran, M, White, SJ, McQuaid, S, Lappin, TR, Thompson, A & James, JA 2014, 'POU2F1 activity regulates HOXD10 and HOXD11 promoting a proliferative and invasive phenotype in Head and Neck cancer', Oncotarget, vol. 5, no. 18, pp. 8803-8815. https://doi.org/10.18632/oncotarget.2492

    POU2F1 activity regulates HOXD10 and HOXD11 promoting a proliferative and invasive phenotype in Head and Neck cancer. / Sharpe, Daniel J.; Orr, Katy S.; Moran, Michael; White, Sharon J.; McQuaid, Stephen; Lappin, Terence R.; Thompson, Alexander; James, Jacqueline A. (Lead / Corresponding author).

    In: Oncotarget, Vol. 5, No. 18, 16.09.2014, p. 8803-8815.

    Research output: Contribution to journalArticle

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    AB - HOX genes are master regulators of organ morphogenesis and cell differentiation during embryonic development, and continue to be expressed throughout post-natal life. To test the hypothesis that HOX genes are dysregulated in head and neck squamous cell carcinoma (HNSCC) we defined their expression profile, and investigated the function, transcriptional regulation and clinical relevance of a subset of highly expressed HOXD genes. Two HOXD genes, D10 and D11, showed strikingly high levels in HNSCC cell lines, patient tumor samples and publicly available datasets. Knockdown of HOXD10 in HNSCC cells caused decreased proliferation and invasion, whereas knockdown of HOXD11 reduced only invasion. POU2F1 consensus sequences were identified in the 5' DNA of HOXD10 and D11. Knockdown of POU2F1 significantly reduced expression of HOXD10 and D11 and inhibited HNSCC proliferation. Luciferase reporter constructs of the HOXD10 and D11 promoters confirmed that POU2F1 consensus binding sites are required for optimal promoter activity. Utilizing patient tumor samples a significant association was found between immunohistochemical staining of HOXD10 and both the overall and the disease-specific survival, adding further support that HOXD10 is dysregulated in head and neck cancer. Additional studies are now warranted to fully evaluate HOXD10 as a prognostic tool in head and neck cancers.

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