Projects per year
Abstract
PP1 and PP2A-B56 are major serine/threonine phosphatase families that achieve specificity by colocalizing with substrates. At the kinetochore, however, both phosphatases localize to an almost identical molecular space and yet they still manage to regulate unique pathways and processes. By switching or modulating the positions of PP1/PP2A-B56 at kinetochores, we show that their unique downstream effects are not due to either the identity of the phosphatase or its precise location. Instead, these phosphatases signal differently because their kinetochore recruitment can be either inhibited (PP1) or enhanced (PP2A) by phosphorylation inputs. Mathematical modeling explains how these inverse phospho-dependencies elicit unique forms of cross-regulation and feedback, which allows otherwise indistinguishable phosphatases to produce distinct network behaviors and control different mitotic processes. Furthermore, our genome-wide analysis suggests that these major phosphatase families may have evolved to respond to phosphorylation inputs in opposite ways because many other PP1 and PP2A-B56-binding motifs are also phospho-regulated.
Original language | English |
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Pages (from-to) | 2206-2219.e8 |
Number of pages | 22 |
Journal | Cell Reports |
Volume | 28 |
Issue number | 8 |
Early online date | 20 Aug 2019 |
DOIs | |
Publication status | Published - 20 Aug 2019 |
Keywords
- Mitosis
- SAC
- SliM
- kinase
- kinetochore
- microtubule attachment
- phosphatase binding
- phosphorylation
- short linear motif
- spindle assembly checkpoint
ASJC Scopus subject areas
- General Biochemistry,Genetics and Molecular Biology
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Dive into the research topics of 'PP1 and PP2A use opposite phospho-dependencies to control distinct processes at the kinetochore'. Together they form a unique fingerprint.Projects
- 1 Finished
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Kinase-Phosphatase Coupling at the Kinetochore and the Maintenance of Chromosomal Stability (joint with University of Edinburgh)
Saurin, A. (Investigator)
1/06/16 → 30/11/24
Project: Research
Student theses
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Investigating the Interplay between Kinases and Phosphatases at the Kinetochore
Smith, R. (Author), Saurin, A. (Supervisor) & Hiom, K. (Supervisor), 2021Student thesis: Doctoral Thesis › Doctor of Philosophy
Profiles
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Saurin, Adrian
- Cancer Research - Professor (Teaching and Research) & Professor of Genome Stability
Person: Academic