ppGpp regulates the binding of two RNA polymerase molecules to the tyrT promoter

Andrew A. Travers, Angus I. Lamond, Hilary A.F. Mace

    Research output: Contribution to journalArticlepeer-review

    15 Citations (Scopus)

    Abstract

    Bacterial promoters differ in the number of RNA polymerase molecules that bind to form a filterable polymerase-promoter complex. We show that two holoenzyme molecules interact with the tyrT promoter, probably as a dimer. This interaction is inhibited by ppGpp. By contrast a single holoenzyme monomer suffices for complex formation at the lacUV5 promoter. We propose that In vivo promoter selection by monomeric and dimeric forms of the enzyme could coordinate the synthesis of stable RNA with that of mRNA and could also account in part for the switch in transcriptiona selectivity during the stringent response.

    Original languageEnglish
    Pages (from-to)5043-5057
    Number of pages15
    JournalNucleic Acids Research
    Volume10
    Issue number16
    DOIs
    Publication statusPublished - 25 Aug 1982

    ASJC Scopus subject areas

    • Genetics

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