TY - CHAP
T1 - Practical approaches to investigate redox regulation of heat shock protein expression and intracellular glutathione redox state
AU - Calabrese, Vittorio
AU - Signorile, Anna
AU - Cornelius, Carolin
AU - Mancuso, Cesare
AU - Scapagnini, Giovanni
AU - Ventimiglia, Bernardo
AU - Ragusa, Nicolo
AU - Dinkova-Kostova, Albena
PY - 2008
Y1 - 2008
N2 - The products of vitagenes such as heat shock protein 32 (Hsp32, heme oxygenase 1) and Hsp70, the family of inducible cytoprotective proteins regulated by the Keap1/Nrf2/ARE pathway, and small molecule antioxidants such as glutathione provide the cell with powerful means to counteract and survive various conditions of stress. Among these protective systems, the heat shock proteins represent a highly conserved and robust way for preservation of correct protein conformation, recovery of damaged proteins, and cell survival. Their regulation is dependent on the redox status of the cell, thus redox regulation is rapidly evolving as an important metabolic modulator of cellular functions, and is being increasingly implicated in many chronic inflammatory and degenerative diseases. Protein thiols play a key role in redox sensing, and regulation of cellular redox state is crucial mediator of multiple metabolic, signalling and transcriptional processes in the brain. Nitric oxide, and reactive nitrogen species induce the transcription of vitagenes and Keap1/Nrf2/ARE-dependent genes whose functional products protect against a wide array of subsequent challenges. Emerging interest is now focusing on exogenous small molecules that are capable of activating these systems as a novel target to minimize deleterious consequences associated with free radical-induced cell damage, such as during neurodegeneration. This chapter describes methods that can be used to assess the expression of heat shock proteins and the cellular glutathione redox status and discusses their relevance to mechanisms modulating the onset and progression of neurodegenerative diseases.
AB - The products of vitagenes such as heat shock protein 32 (Hsp32, heme oxygenase 1) and Hsp70, the family of inducible cytoprotective proteins regulated by the Keap1/Nrf2/ARE pathway, and small molecule antioxidants such as glutathione provide the cell with powerful means to counteract and survive various conditions of stress. Among these protective systems, the heat shock proteins represent a highly conserved and robust way for preservation of correct protein conformation, recovery of damaged proteins, and cell survival. Their regulation is dependent on the redox status of the cell, thus redox regulation is rapidly evolving as an important metabolic modulator of cellular functions, and is being increasingly implicated in many chronic inflammatory and degenerative diseases. Protein thiols play a key role in redox sensing, and regulation of cellular redox state is crucial mediator of multiple metabolic, signalling and transcriptional processes in the brain. Nitric oxide, and reactive nitrogen species induce the transcription of vitagenes and Keap1/Nrf2/ARE-dependent genes whose functional products protect against a wide array of subsequent challenges. Emerging interest is now focusing on exogenous small molecules that are capable of activating these systems as a novel target to minimize deleterious consequences associated with free radical-induced cell damage, such as during neurodegeneration. This chapter describes methods that can be used to assess the expression of heat shock proteins and the cellular glutathione redox status and discusses their relevance to mechanisms modulating the onset and progression of neurodegenerative diseases.
KW - NITRIC-OXIDE SYNTHASE
KW - CELLULAR STRESS-RESPONSE
KW - KAPPA-B ACTIVATION
KW - HEME OXYGENASE-1 INDUCTION
KW - COLORECTAL-CARCINOMA CELLS
KW - VASCULAR ENDOTHELIAL-CELLS
KW - HSP70 GENE-EXPRESSION
KW - RAT GLIAL-CELLS
KW - OXIDATIVE-STRESS
KW - NEURODEGENERATIVE DISORDERS
UR - http://www.scopus.com/inward/record.url?scp=52249099052&partnerID=8YFLogxK
U2 - 10.1016/S0076-6879(08)01206-8
DO - 10.1016/S0076-6879(08)01206-8
M3 - Other chapter contribution
SN - 9780123743091
T3 - Methods in Enzymology
SP - 83
EP - 110
BT - Nitric oxide, Part G oxidative and nitrosative stress in redox regulation of cell signaling
A2 - Cadenas, Enrique
A2 - Packer, Lester
PB - Academic Press
CY - San Diego
ER -