PRCC, the commonest TFE3 fusion partner in papillary renal carcinoma is associated with pre-mRNA splicing factors

Y. M. Skalsky, P. M. Ajuh, C. Parker, A. I. Lamond, G. Goodwin, C. S. Cooper

    Research output: Contribution to journalArticle

    51 Citations (Scopus)

    Abstract

    In papillary renal cell carcinomas the TFE3 transcription factor becomes fused to the PSF and NonO pre-mRNA splicing factors and most commonly to a protein of unknown function designated PRCC. In this study we have examined the ability of the resulting PRCC-TFE3 and NonO-TFE3 fusions to activate transcription from the plasminogen activator inhibitor-1 (PAI-1) promoter. The results show that only fusion to PRCC enhanced transcriptional activation, indicating that the ability to enhance the level of transcription from endogenous TFE3 promoters is not a consistent feature of TFE3 fusions. In investigations of the normal function of PRCC we observed that PRCC expressed as a green fluorescent fusion protein colocalizes within the nucleus with Sm pre-mRNA splicing factors. It was also found that endogenous PRCC is coimmunoprecipitated by antibodies that recognize a variety of pre-mRNA splicing factors including SC35, PRL1 and CDC5. Association with the cellular splicing machinery is therefore, a common feature of the proteins that become fused to TFE3 in papillary renal cell carcinomas.

    Original languageEnglish
    Pages (from-to)178-187
    Number of pages10
    JournalOncogene
    Volume20
    Issue number2
    DOIs
    Publication statusPublished - 25 Dec 2000

    Keywords

    • PRCC
    • Pre-mRNA splicing
    • TFE3
    • Transcription activation

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