Preclinical and clinical evaluation of sulforaphane for chemoprevention in the breast

Brian S. Cornblatt, Lingxiang Ye, Albena T. Dinkova-Kostova, Melanie Erb, Jed W. Fahey, Navin K. Singh, Min Shue A. Chen, Tracey Stierer, Elizabeth Garrett-Mayer, Pedram Argani, Nancy E. Davidson, Paul Talalay, Thomas W. Kensler, Kala Visvanathan (Lead / Corresponding author)

Research output: Contribution to journalArticle

212 Citations (Scopus)

Abstract

Consumers of higher levels of Brassica vegetables, particularly those of the genus Brassica (broccoli, Brussels sprouts and cabbage), reduce their susceptibility to cancer at a variety of organ sites. Brassica vegetables contain high concentrations of glucosinolates that can be hydrolyzed by the plant enzyme, myrosinase, or intestinal microflora to isothiocyanates, potent inducers of cytoprotective enzymes and inhibitors of carcinogenesis. Oral administration of either the isothiocyanate, sulforaphane, or its glucosinolate precursor, glucoraphanin, inhibits mammary carcinogenesis in rats treated with 7,12-dimethylbenz[a]anthracene. In this study, we sought to determine whether sulforaphane exerts a direct chemopreventive action on animal and human mammary tissue. The pharmacokinetics and pharmacodynamics of a single 150 μmol oral dose of sulforaphane were evaluated in the rat mammary gland. We detected sulforaphane metabolites at concentrations known to alter gene expression in cell culture. Elevated cytoprotective NAD(P)H:quinone oxidoreductase (NQO1) and heme oxygenase-1 (HO-1) gene transcripts were measured using quantitative real-time polymerase chain reaction. An observed 3-fold increase in NQO1 enzymatic activity, as well as 4-fold elevated immunostaining of HO-1 in rat mammary epithelium, provides strong evidence of a pronounced pharmacodynamic action of sulforaphane. In a subsequent pilot study, eight healthy women undergoing reduction mammoplasty were given a single dose of a broccoli sprout preparation containing 200 μmol of sulforaphane. Following oral dosing, sulforaphane metabolites were readily measurable in human breast tissue enriched for epithelial cells. These findings provide a strong rationale for evaluating the protective effects of a broccoli sprout preparation in clinical trials of women at risk for breast cancer.

Original languageEnglish
Pages (from-to)1485-1490
Number of pages6
JournalCarcinogenesis
Volume28
Issue number7
DOIs
Publication statusPublished - 1 Jul 2007

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Brassica
Chemoprevention
Breast
Glucosinolates
Heme Oxygenase-1
Vegetables
Carcinogenesis
Isothiocyanates
Mammaplasty
Enzyme Inhibitors
Human Mammary Glands
sulforafan
NAD
Oral Administration
Real-Time Polymerase Chain Reaction
Oxidoreductases
Epithelium
Pharmacokinetics
Cell Culture Techniques
Epithelial Cells

Cite this

Cornblatt, B. S., Ye, L., Dinkova-Kostova, A. T., Erb, M., Fahey, J. W., Singh, N. K., ... Visvanathan, K. (2007). Preclinical and clinical evaluation of sulforaphane for chemoprevention in the breast. Carcinogenesis, 28(7), 1485-1490. https://doi.org/10.1093/carcin/bgm049
Cornblatt, Brian S. ; Ye, Lingxiang ; Dinkova-Kostova, Albena T. ; Erb, Melanie ; Fahey, Jed W. ; Singh, Navin K. ; Chen, Min Shue A. ; Stierer, Tracey ; Garrett-Mayer, Elizabeth ; Argani, Pedram ; Davidson, Nancy E. ; Talalay, Paul ; Kensler, Thomas W. ; Visvanathan, Kala. / Preclinical and clinical evaluation of sulforaphane for chemoprevention in the breast. In: Carcinogenesis. 2007 ; Vol. 28, No. 7. pp. 1485-1490.
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Cornblatt, BS, Ye, L, Dinkova-Kostova, AT, Erb, M, Fahey, JW, Singh, NK, Chen, MSA, Stierer, T, Garrett-Mayer, E, Argani, P, Davidson, NE, Talalay, P, Kensler, TW & Visvanathan, K 2007, 'Preclinical and clinical evaluation of sulforaphane for chemoprevention in the breast', Carcinogenesis, vol. 28, no. 7, pp. 1485-1490. https://doi.org/10.1093/carcin/bgm049

Preclinical and clinical evaluation of sulforaphane for chemoprevention in the breast. / Cornblatt, Brian S.; Ye, Lingxiang; Dinkova-Kostova, Albena T.; Erb, Melanie; Fahey, Jed W.; Singh, Navin K.; Chen, Min Shue A.; Stierer, Tracey; Garrett-Mayer, Elizabeth; Argani, Pedram; Davidson, Nancy E.; Talalay, Paul; Kensler, Thomas W.; Visvanathan, Kala (Lead / Corresponding author).

In: Carcinogenesis, Vol. 28, No. 7, 01.07.2007, p. 1485-1490.

Research output: Contribution to journalArticle

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AU - Cornblatt, Brian S.

AU - Ye, Lingxiang

AU - Dinkova-Kostova, Albena T.

AU - Erb, Melanie

AU - Fahey, Jed W.

AU - Singh, Navin K.

AU - Chen, Min Shue A.

AU - Stierer, Tracey

AU - Garrett-Mayer, Elizabeth

AU - Argani, Pedram

AU - Davidson, Nancy E.

AU - Talalay, Paul

AU - Kensler, Thomas W.

AU - Visvanathan, Kala

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N2 - Consumers of higher levels of Brassica vegetables, particularly those of the genus Brassica (broccoli, Brussels sprouts and cabbage), reduce their susceptibility to cancer at a variety of organ sites. Brassica vegetables contain high concentrations of glucosinolates that can be hydrolyzed by the plant enzyme, myrosinase, or intestinal microflora to isothiocyanates, potent inducers of cytoprotective enzymes and inhibitors of carcinogenesis. Oral administration of either the isothiocyanate, sulforaphane, or its glucosinolate precursor, glucoraphanin, inhibits mammary carcinogenesis in rats treated with 7,12-dimethylbenz[a]anthracene. In this study, we sought to determine whether sulforaphane exerts a direct chemopreventive action on animal and human mammary tissue. The pharmacokinetics and pharmacodynamics of a single 150 μmol oral dose of sulforaphane were evaluated in the rat mammary gland. We detected sulforaphane metabolites at concentrations known to alter gene expression in cell culture. Elevated cytoprotective NAD(P)H:quinone oxidoreductase (NQO1) and heme oxygenase-1 (HO-1) gene transcripts were measured using quantitative real-time polymerase chain reaction. An observed 3-fold increase in NQO1 enzymatic activity, as well as 4-fold elevated immunostaining of HO-1 in rat mammary epithelium, provides strong evidence of a pronounced pharmacodynamic action of sulforaphane. In a subsequent pilot study, eight healthy women undergoing reduction mammoplasty were given a single dose of a broccoli sprout preparation containing 200 μmol of sulforaphane. Following oral dosing, sulforaphane metabolites were readily measurable in human breast tissue enriched for epithelial cells. These findings provide a strong rationale for evaluating the protective effects of a broccoli sprout preparation in clinical trials of women at risk for breast cancer.

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