Preparation data of the bromodomains BRD3(1), BRD3(2), BRD4(1), and BRPF1B and crystallization of BRD4(1)-inhibitor complexes

Martin Hügle; Xavier Lucas; Gerhard Weitzel; Dmytro Ostrovskyi; Bernhard Breit; Stefan Gerhardt; Karin Schmidtkunz; Manfred Jung; Roland Schüle; Oliver Einsle; Stefan Günther; Daniel Wohlwend

doi:10.1016/j.dib.2016.04.009

Preparation data of the bromodomains BRD3(1), BRD3(2), BRD4(1), and BRPF1B and crystallization of BRD4(1)-inhibitor complexes

Martin Hügle
, Xavier Lucas
, Gerhard Weitzel
, Dmytro Ostrovskyi
, Bernhard Breit
, Stefan Gerhardt
, Karin Schmidtkunz
, Manfred Jung
, Roland Schüle
, Oliver Einsle
, Stefan Günther
, Daniel Wohlwend (Lead / Corresponding author)

Biological Chemistry and Drug Discovery

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

377 Downloads (Pure)

Abstract

This article presents detailed purification procedures for the bromodomains BRD3(1), BRD3(2), BRD4(1), and BRPF1B. In addition we provide crystallization protocols for apo BRD4(1) and BRD4(1) in complex with numerous inhibitors. The protocols described here were successfully applied to obtain affinity data by isothermal titration calorimetry (ITC) and by differential scanning fluorimetry (DSF) as well as structural characterizations of BRD4(1) inhibitor complexes (PDB codes: PDB: 4LYI, PDB: 4LZS, PDB: 4LYW, PDB: 4LZR, PDB: 4LYS, PDB: 5D24, PDB: 5D25, PDB: 5D26, PDB: 5D3H, PDB: 5D3J, PDB: 5D3L, PDB: 5D3N, PDB: 5D3P, PDB: 5D3R, PDB: 5D3S, PDB: 5D3T). These data have been reported previously and are discussed in more detail elsewhere [1,2].

Original language	English
Pages (from-to)	1370-1374
Number of pages	5
Journal	Data in Brief
Volume	7
Early online date	11 Apr 2016
DOIs	https://doi.org/10.1016/j.dib.2016.04.009
Publication status	Published - 1 Jun 2016

Keywords

Bromodomains
Drug discovery
Epigenetics
X-ray crystallography

ASJC Scopus subject areas

General
Education

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10.1016/j.dib.2016.04.009Licence: CC BY

Publisher final version
2352-3409/copyright 2016 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Final published version, 684 KBLicence: CC BY

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@article{b7bcce00873d42989132779c959ec317,

title = "Preparation data of the bromodomains BRD3(1), BRD3(2), BRD4(1), and BRPF1B and crystallization of BRD4(1)-inhibitor complexes",

abstract = "This article presents detailed purification procedures for the bromodomains BRD3(1), BRD3(2), BRD4(1), and BRPF1B. In addition we provide crystallization protocols for apo BRD4(1) and BRD4(1) in complex with numerous inhibitors. The protocols described here were successfully applied to obtain affinity data by isothermal titration calorimetry (ITC) and by differential scanning fluorimetry (DSF) as well as structural characterizations of BRD4(1) inhibitor complexes (PDB codes: PDB: 4LYI, PDB: 4LZS, PDB: 4LYW, PDB: 4LZR, PDB: 4LYS, PDB: 5D24, PDB: 5D25, PDB: 5D26, PDB: 5D3H, PDB: 5D3J, PDB: 5D3L, PDB: 5D3N, PDB: 5D3P, PDB: 5D3R, PDB: 5D3S, PDB: 5D3T). These data have been reported previously and are discussed in more detail elsewhere [1,2].",

keywords = "Bromodomains, Drug discovery, Epigenetics, X-ray crystallography",

author = "Martin H{\"u}gle and Xavier Lucas and Gerhard Weitzel and Dmytro Ostrovskyi and Bernhard Breit and Stefan Gerhardt and Karin Schmidtkunz and Manfred Jung and Roland Sch{\"u}le and Oliver Einsle and Stefan G{\"u}nther and Daniel Wohlwend",

year = "2016",

month = jun,

day = "1",

doi = "10.1016/j.dib.2016.04.009",

language = "English",

volume = "7",

pages = "1370--1374",

journal = "Data in Brief",

publisher = "Elsevier",

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TY - JOUR

T1 - Preparation data of the bromodomains BRD3(1), BRD3(2), BRD4(1), and BRPF1B and crystallization of BRD4(1)-inhibitor complexes

AU - Hügle, Martin

AU - Lucas, Xavier

AU - Weitzel, Gerhard

AU - Ostrovskyi, Dmytro

AU - Breit, Bernhard

AU - Gerhardt, Stefan

AU - Schmidtkunz, Karin

AU - Jung, Manfred

AU - Schüle, Roland

AU - Einsle, Oliver

AU - Günther, Stefan

AU - Wohlwend, Daniel

PY - 2016/6/1

Y1 - 2016/6/1

N2 - This article presents detailed purification procedures for the bromodomains BRD3(1), BRD3(2), BRD4(1), and BRPF1B. In addition we provide crystallization protocols for apo BRD4(1) and BRD4(1) in complex with numerous inhibitors. The protocols described here were successfully applied to obtain affinity data by isothermal titration calorimetry (ITC) and by differential scanning fluorimetry (DSF) as well as structural characterizations of BRD4(1) inhibitor complexes (PDB codes: PDB: 4LYI, PDB: 4LZS, PDB: 4LYW, PDB: 4LZR, PDB: 4LYS, PDB: 5D24, PDB: 5D25, PDB: 5D26, PDB: 5D3H, PDB: 5D3J, PDB: 5D3L, PDB: 5D3N, PDB: 5D3P, PDB: 5D3R, PDB: 5D3S, PDB: 5D3T). These data have been reported previously and are discussed in more detail elsewhere [1,2].

AB - This article presents detailed purification procedures for the bromodomains BRD3(1), BRD3(2), BRD4(1), and BRPF1B. In addition we provide crystallization protocols for apo BRD4(1) and BRD4(1) in complex with numerous inhibitors. The protocols described here were successfully applied to obtain affinity data by isothermal titration calorimetry (ITC) and by differential scanning fluorimetry (DSF) as well as structural characterizations of BRD4(1) inhibitor complexes (PDB codes: PDB: 4LYI, PDB: 4LZS, PDB: 4LYW, PDB: 4LZR, PDB: 4LYS, PDB: 5D24, PDB: 5D25, PDB: 5D26, PDB: 5D3H, PDB: 5D3J, PDB: 5D3L, PDB: 5D3N, PDB: 5D3P, PDB: 5D3R, PDB: 5D3S, PDB: 5D3T). These data have been reported previously and are discussed in more detail elsewhere [1,2].

KW - Bromodomains

KW - Drug discovery

KW - Epigenetics

KW - X-ray crystallography

UR - https://www.scopus.com/pages/publications/84963819015

U2 - 10.1016/j.dib.2016.04.009

DO - 10.1016/j.dib.2016.04.009

M3 - Article

C2 - 27158652

AN - SCOPUS:84963819015

VL - 7

SP - 1370

EP - 1374

JO - Data in Brief

JF - Data in Brief

ER -

Preparation data of the bromodomains BRD3(1), BRD3(2), BRD4(1), and BRPF1B and crystallization of BRD4(1)-inhibitor complexes

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