Projects per year
Abstract
Recombination (crossover) drives the release of genetic diversity in plant breeding programs. However, in barley, recombination is skewed toward the telomeric ends of its seven chromosomes, restricting the re-assortment of about 30% of the genes located in the centromeric regions of its large 5.1 Gb genome. A better understanding of meiosis and recombination could provide ways of modulating crossover distribution and frequency in barley as well as in other grasses, including wheat. While most research on recombination has been carried out in the model plant Arabidopsis thaliana, recent studies in barley (Hordeum Vulgare) have provided new insights into the control of crossing over in large genome species. A major achievement in these studies has been the use of cytological procedures to follow meiotic events. This protocol provides detailed practical steps required to perform immunostaining of barley meiocytes (pollen mother cells) for confocal or structured illumination microscopy.
Original language | English |
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Title of host publication | Barley |
Subtitle of host publication | Methods and Protocols |
Editors | Wendy A. Harwood |
Place of Publication | New York |
Publisher | Humana Press |
Pages | 167-179 |
Number of pages | 13 |
Volume | 1900 |
ISBN (Electronic) | 9781493989447 |
ISBN (Print) | 9781493989423 |
DOIs | |
Publication status | Published - 2019 |
Publication series
Name | Methods in Molecular Biology |
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Publisher | Springer |
ISSN (Print) | 1064-3745 |
Keywords
- 3D-SIM
- Antibodies
- Barley
- Confocal Microscopy
- Immuno-cytochemistry
- Meiosis
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Fingerprint
Dive into the research topics of 'Preparation of Barley Pollen Mother Cells for Confocal and Super Resolution Microscopy'. Together they form a unique fingerprint.Projects
- 1 Finished
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Dynamics of Fundamental Cellular Processes by Live Cell and Tissue Imaging
MacDonald, M. (Investigator), McGloin, D. (Investigator), McKenna, S. (Investigator), Storey, K. (Investigator), Swedlow, J. (Investigator) & Weijer, K. (Investigator)
1/01/13 → 31/12/17
Project: Research
Student theses
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Discovery of a novel meiotic E3 ubiquitin ligase by characterisation of the barley desynaptic mutant DES12.W
Mittmann, S. U. (Author), Waugh, R. (Supervisor), Colas, I. (Supervisor) & Ramsay, L. (Supervisor), 2017Student thesis: Doctoral Thesis › Doctor of Philosophy
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