Preparation of Barley Pollen Mother Cells for Confocal and Super Resolution Microscopy

Sybille Mittmann, Mikel Arrieta, Luke Ramsay, Robbie Waugh, Isabelle Colas (Lead / Corresponding author)

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Abstract

Recombination (crossover) drives the release of genetic diversity in plant breeding programs. However, in barley, recombination is skewed toward the telomeric ends of its seven chromosomes, restricting the re-assortment of about 30% of the genes located in the centromeric regions of its large 5.1 Gb genome. A better understanding of meiosis and recombination could provide ways of modulating crossover distribution and frequency in barley as well as in other grasses, including wheat. While most research on recombination has been carried out in the model plant Arabidopsis thaliana, recent studies in barley (Hordeum Vulgare) have provided new insights into the control of crossing over in large genome species. A major achievement in these studies has been the use of cytological procedures to follow meiotic events. This protocol provides detailed practical steps required to perform immunostaining of barley meiocytes (pollen mother cells) for confocal or structured illumination microscopy.

Original languageEnglish
Title of host publicationBarley
Subtitle of host publicationMethods and Protocols
EditorsWendy A. Harwood
Place of PublicationNew York
PublisherHumana Press
Pages167-179
Number of pages13
Volume1900
ISBN (Electronic)9781493989447
ISBN (Print)9781493989423
DOIs
Publication statusPublished - 2019

Publication series

NameMethods in Molecular Biology
PublisherSpringer
ISSN (Print)1064-3745

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Keywords

  • 3D-SIM
  • Antibodies
  • Barley
  • Confocal Microscopy
  • Immuno-cytochemistry
  • Meiosis

Cite this

Mittmann, S., Arrieta, M., Ramsay, L., Waugh, R., & Colas, I. (2019). Preparation of Barley Pollen Mother Cells for Confocal and Super Resolution Microscopy. In W. A. Harwood (Ed.), Barley: Methods and Protocols (Vol. 1900, pp. 167-179). (Methods in Molecular Biology). Humana Press. https://doi.org/10.1007/978-1-4939-8944-7_11