Preparation of transition-state analogues of sterol 24-methyl transferase as potential anti-parasities

S O Lorente, C J Jimenez, L Gros, V Yardley, K de Luca-Fradley, S L Croft, J A Urbina, L M Ruiz-Perez, D G Pacanowska, I H Gilbert

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    Abstract

    There is an urgent need for new drugs to treat leishmaniasis and Chagas disease. One important drug target in these organisms is sterol biosynthesis. In these organisms the main endogenous sterols are ergosta- and stigmata-like compounds in contrast to the situation in mammals, which have cholesterol as the sole sterol. In this paper we discuss the design, synthesis and evaluation of potential transition state analogues of the enzyme Delta(24(25))-methyltransferase (24-SMT). This enzyme is essential for the biosynthesis of ergosterol, but not required for the biosynthesis of cholesterol. A series of compounds were successfully synthesised in which mimics of the S-adenosyl methionine co-factor were attached to the sterol nucleus. Compounds were evaluated against recombinant Leishmania major 24-SMT and the parasites L. donovani and Trypanosoma cruzi in vitro, causative organisms of leishmaniasis and Chagas disease, respectively. Some of the-compounds showed inhibition of the recombinant Leishmania major 24-SMT and induced growth inhibition of the parasites. Some compounds also showed anti-parasitic activity against L. donovani and T cruzi, but no inhibition of the enzyme. In addition, some of the compounds had anti-proliferative activity against the bloodstream forms of Trypanosoma brucei rhodesiense, which causes African trypanosomiasis.

    Original languageEnglish
    Pages (from-to)5435-5453
    Number of pages19
    JournalBioorganic & Medicinal Chemistry
    Volume13
    Issue number18
    DOIs
    Publication statusPublished - 15 Sep 2005

    Cite this

    Lorente, S O ; Jimenez, C J ; Gros, L ; Yardley, V ; de Luca-Fradley, K ; Croft, S L ; Urbina, J A ; Ruiz-Perez, L M ; Pacanowska, D G ; Gilbert, I H . / Preparation of transition-state analogues of sterol 24-methyl transferase as potential anti-parasities. In: Bioorganic & Medicinal Chemistry. 2005 ; Vol. 13, No. 18. pp. 5435-5453.
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    abstract = "There is an urgent need for new drugs to treat leishmaniasis and Chagas disease. One important drug target in these organisms is sterol biosynthesis. In these organisms the main endogenous sterols are ergosta- and stigmata-like compounds in contrast to the situation in mammals, which have cholesterol as the sole sterol. In this paper we discuss the design, synthesis and evaluation of potential transition state analogues of the enzyme Delta(24(25))-methyltransferase (24-SMT). This enzyme is essential for the biosynthesis of ergosterol, but not required for the biosynthesis of cholesterol. A series of compounds were successfully synthesised in which mimics of the S-adenosyl methionine co-factor were attached to the sterol nucleus. Compounds were evaluated against recombinant Leishmania major 24-SMT and the parasites L. donovani and Trypanosoma cruzi in vitro, causative organisms of leishmaniasis and Chagas disease, respectively. Some of the-compounds showed inhibition of the recombinant Leishmania major 24-SMT and induced growth inhibition of the parasites. Some compounds also showed anti-parasitic activity against L. donovani and T cruzi, but no inhibition of the enzyme. In addition, some of the compounds had anti-proliferative activity against the bloodstream forms of Trypanosoma brucei rhodesiense, which causes African trypanosomiasis.",
    author = "Lorente, {S O} and Jimenez, {C J} and L Gros and V Yardley and {de Luca-Fradley}, K and Croft, {S L} and Urbina, {J A} and Ruiz-Perez, {L M} and Pacanowska, {D G} and Gilbert, {I H}",
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    Lorente, SO, Jimenez, CJ, Gros, L, Yardley, V, de Luca-Fradley, K, Croft, SL, Urbina, JA, Ruiz-Perez, LM, Pacanowska, DG & Gilbert, IH 2005, 'Preparation of transition-state analogues of sterol 24-methyl transferase as potential anti-parasities', Bioorganic & Medicinal Chemistry, vol. 13, no. 18, pp. 5435-5453. https://doi.org/10.1016/j.bmc.2005.06.012

    Preparation of transition-state analogues of sterol 24-methyl transferase as potential anti-parasities. / Lorente, S O ; Jimenez, C J ; Gros, L ; Yardley, V ; de Luca-Fradley, K ; Croft, S L ; Urbina, J A ; Ruiz-Perez, L M ; Pacanowska, D G ; Gilbert, I H .

    In: Bioorganic & Medicinal Chemistry, Vol. 13, No. 18, 15.09.2005, p. 5435-5453.

    Research output: Contribution to journalArticle

    TY - JOUR

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    AU - Lorente, S O

    AU - Jimenez, C J

    AU - Gros, L

    AU - Yardley, V

    AU - de Luca-Fradley, K

    AU - Croft, S L

    AU - Urbina, J A

    AU - Ruiz-Perez, L M

    AU - Pacanowska, D G

    AU - Gilbert, I H

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    N2 - There is an urgent need for new drugs to treat leishmaniasis and Chagas disease. One important drug target in these organisms is sterol biosynthesis. In these organisms the main endogenous sterols are ergosta- and stigmata-like compounds in contrast to the situation in mammals, which have cholesterol as the sole sterol. In this paper we discuss the design, synthesis and evaluation of potential transition state analogues of the enzyme Delta(24(25))-methyltransferase (24-SMT). This enzyme is essential for the biosynthesis of ergosterol, but not required for the biosynthesis of cholesterol. A series of compounds were successfully synthesised in which mimics of the S-adenosyl methionine co-factor were attached to the sterol nucleus. Compounds were evaluated against recombinant Leishmania major 24-SMT and the parasites L. donovani and Trypanosoma cruzi in vitro, causative organisms of leishmaniasis and Chagas disease, respectively. Some of the-compounds showed inhibition of the recombinant Leishmania major 24-SMT and induced growth inhibition of the parasites. Some compounds also showed anti-parasitic activity against L. donovani and T cruzi, but no inhibition of the enzyme. In addition, some of the compounds had anti-proliferative activity against the bloodstream forms of Trypanosoma brucei rhodesiense, which causes African trypanosomiasis.

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    U2 - 10.1016/j.bmc.2005.06.012

    DO - 10.1016/j.bmc.2005.06.012

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    JO - Bioorganic & Medicinal Chemistry

    JF - Bioorganic & Medicinal Chemistry

    SN - 0968-0896

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