Presence of Epstein-Barr virus in Hodgkin's disease is not exclusive to Reed-Sternberg cells

G. Khan, P. J. Coates, R. K. Gupta, H. O. Kangro, G. Slavin

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    Abstract

    Thirty-three cases of Hodgkin's disease (HD) have been studied for the presence of Epstein-Barr virus (EBV) using a novel nonisotopic in situ hybridization procedure, based on the detection of Epstein-Barr encoded RNAs with oligonucleotide probes. An intense and morphologically distinct nuclear staining, sparing the nucleolus was seen in a total of 12 cases (36%). In six of these cases, the signal was located to the Hodgkin and Reed-Sternberg cells (HR-S); in the other six positive cases, the signal was observed only in the non-neoplastic small lymphocytes. These lymphocytes were few in number and immunocytochemistry results were consistent with a B-cell phenotype. The presence of EBV in those cases characterized by nuclear staining of small lymphocytes was confirmed by the polymerase chain reaction (PCR) analysis. The authors report the detection of EBV in small lymphocytes in HD by in situ hybridization and discuss the implications of these findings in relation to the proposed etiologic association between EBV and HD.
    Original languageEnglish
    Pages (from-to)757-762
    Number of pages6
    JournalAmerican Journal of Pathology
    Volume140
    Issue number4
    Publication statusPublished - 1992

    Fingerprint

    Reed-Sternberg Cells
    Human Herpesvirus 4
    Hodgkin Disease
    Lymphocytes
    In Situ Hybridization
    Staining and Labeling
    Oligonucleotide Probes
    Virus Diseases
    B-Lymphocytes
    Immunohistochemistry
    RNA
    Phenotype
    Polymerase Chain Reaction

    Cite this

    Khan, G., Coates, P. J., Gupta, R. K., Kangro, H. O., & Slavin, G. (1992). Presence of Epstein-Barr virus in Hodgkin's disease is not exclusive to Reed-Sternberg cells. American Journal of Pathology, 140(4), 757-762.
    Khan, G. ; Coates, P. J. ; Gupta, R. K. ; Kangro, H. O. ; Slavin, G. / Presence of Epstein-Barr virus in Hodgkin's disease is not exclusive to Reed-Sternberg cells. In: American Journal of Pathology. 1992 ; Vol. 140, No. 4. pp. 757-762.
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    abstract = "Thirty-three cases of Hodgkin's disease (HD) have been studied for the presence of Epstein-Barr virus (EBV) using a novel nonisotopic in situ hybridization procedure, based on the detection of Epstein-Barr encoded RNAs with oligonucleotide probes. An intense and morphologically distinct nuclear staining, sparing the nucleolus was seen in a total of 12 cases (36{\%}). In six of these cases, the signal was located to the Hodgkin and Reed-Sternberg cells (HR-S); in the other six positive cases, the signal was observed only in the non-neoplastic small lymphocytes. These lymphocytes were few in number and immunocytochemistry results were consistent with a B-cell phenotype. The presence of EBV in those cases characterized by nuclear staining of small lymphocytes was confirmed by the polymerase chain reaction (PCR) analysis. The authors report the detection of EBV in small lymphocytes in HD by in situ hybridization and discuss the implications of these findings in relation to the proposed etiologic association between EBV and HD.",
    author = "G. Khan and Coates, {P. J.} and Gupta, {R. K.} and Kangro, {H. O.} and G. Slavin",
    year = "1992",
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    volume = "140",
    pages = "757--762",
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    Khan, G, Coates, PJ, Gupta, RK, Kangro, HO & Slavin, G 1992, 'Presence of Epstein-Barr virus in Hodgkin's disease is not exclusive to Reed-Sternberg cells', American Journal of Pathology, vol. 140, no. 4, pp. 757-762.

    Presence of Epstein-Barr virus in Hodgkin's disease is not exclusive to Reed-Sternberg cells. / Khan, G.; Coates, P. J.; Gupta, R. K.; Kangro, H. O.; Slavin, G.

    In: American Journal of Pathology, Vol. 140, No. 4, 1992, p. 757-762.

    Research output: Contribution to journalArticle

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    T1 - Presence of Epstein-Barr virus in Hodgkin's disease is not exclusive to Reed-Sternberg cells

    AU - Khan, G.

    AU - Coates, P. J.

    AU - Gupta, R. K.

    AU - Kangro, H. O.

    AU - Slavin, G.

    PY - 1992

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    N2 - Thirty-three cases of Hodgkin's disease (HD) have been studied for the presence of Epstein-Barr virus (EBV) using a novel nonisotopic in situ hybridization procedure, based on the detection of Epstein-Barr encoded RNAs with oligonucleotide probes. An intense and morphologically distinct nuclear staining, sparing the nucleolus was seen in a total of 12 cases (36%). In six of these cases, the signal was located to the Hodgkin and Reed-Sternberg cells (HR-S); in the other six positive cases, the signal was observed only in the non-neoplastic small lymphocytes. These lymphocytes were few in number and immunocytochemistry results were consistent with a B-cell phenotype. The presence of EBV in those cases characterized by nuclear staining of small lymphocytes was confirmed by the polymerase chain reaction (PCR) analysis. The authors report the detection of EBV in small lymphocytes in HD by in situ hybridization and discuss the implications of these findings in relation to the proposed etiologic association between EBV and HD.

    AB - Thirty-three cases of Hodgkin's disease (HD) have been studied for the presence of Epstein-Barr virus (EBV) using a novel nonisotopic in situ hybridization procedure, based on the detection of Epstein-Barr encoded RNAs with oligonucleotide probes. An intense and morphologically distinct nuclear staining, sparing the nucleolus was seen in a total of 12 cases (36%). In six of these cases, the signal was located to the Hodgkin and Reed-Sternberg cells (HR-S); in the other six positive cases, the signal was observed only in the non-neoplastic small lymphocytes. These lymphocytes were few in number and immunocytochemistry results were consistent with a B-cell phenotype. The presence of EBV in those cases characterized by nuclear staining of small lymphocytes was confirmed by the polymerase chain reaction (PCR) analysis. The authors report the detection of EBV in small lymphocytes in HD by in situ hybridization and discuss the implications of these findings in relation to the proposed etiologic association between EBV and HD.

    M3 - Article

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