Primary structure of the site on bovine hormone-sensitive lipase phosphorylated by cyclic AMP-dependent protein kinase

Andrew J. Garton; David G. Campbell; Philip Cohen; Stephen J. Yeaman

doi:10.1016/0014-5793(88)80799-3

Primary structure of the site on bovine hormone-sensitive lipase phosphorylated by cyclic AMP-dependent protein kinase

Andrew J. Garton, David G. Campbell, Philip Cohen, Stephen J. Yeaman

MRC PPU

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Abstract

The primary structure of a region on hormone-sensitive lipase was determined to be: Lys-Thr-Glu-Pro-Met-Arg-Arg-Ser-Val-Ser-Glu-Ala-Ala-Leu-Thr-Gln-Pro-Glu-Gly-Pro-Leu-Gly-Thr-Asp-Ser-Leu-Lys. Ser-8 was the only residue in the intact protein phosphorylated by cyclic AMP-dependent protein kinase. However, Ser-10 also appeared to be present in a phosphorylated form, suggesting that it is a target for a distinct protein kinase in vivo.

Original language	English
Pages (from-to)	68-72
Number of pages	5
Journal	FEBS Letters
Volume	229
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(88)80799-3
Publication status	Published - 29 Feb 1988

Keywords

Amino acid sequence
cyclic AMP
Hormone-sensitive lipase
Protein kinase
Protein phosphorylation

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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abstract = "The primary structure of a region on hormone-sensitive lipase was determined to be: Lys-Thr-Glu-Pro-Met-Arg-Arg-Ser-Val-Ser-Glu-Ala-Ala-Leu-Thr-Gln-Pro-Glu-Gly-Pro-Leu-Gly-Thr-Asp-Ser-Leu-Lys. Ser-8 was the only residue in the intact protein phosphorylated by cyclic AMP-dependent protein kinase. However, Ser-10 also appeared to be present in a phosphorylated form, suggesting that it is a target for a distinct protein kinase in vivo.",

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AU - Garton, Andrew J.

AU - Campbell, David G.

AU - Cohen, Philip

AU - Yeaman, Stephen J.

PY - 1988/2/29

Y1 - 1988/2/29

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AB - The primary structure of a region on hormone-sensitive lipase was determined to be: Lys-Thr-Glu-Pro-Met-Arg-Arg-Ser-Val-Ser-Glu-Ala-Ala-Leu-Thr-Gln-Pro-Glu-Gly-Pro-Leu-Gly-Thr-Asp-Ser-Leu-Lys. Ser-8 was the only residue in the intact protein phosphorylated by cyclic AMP-dependent protein kinase. However, Ser-10 also appeared to be present in a phosphorylated form, suggesting that it is a target for a distinct protein kinase in vivo.

KW - Amino acid sequence

KW - cyclic AMP

KW - Hormone-sensitive lipase

KW - Protein kinase

KW - Protein phosphorylation

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Primary structure of the site on bovine hormone-sensitive lipase phosphorylated by cyclic AMP-dependent protein kinase

Abstract

Keywords

ASJC Scopus subject areas

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