Probing the Role of Cyclic di-GMP Signaling Systems in Disease Using Chinese Radish

Shi-Qi An, Ji-Liang Tang, John Maxwell Dow (Lead / Corresponding author)

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

7 Citations (Scopus)


The determination of the genome sequences of pathogenic bacteria has facilitated functional analyses that aim to understand the molecular basis of virulence. In particular, genome sequence information of the pathogen Xanthomonas campestris pathovar campestris has allowed researchers to identify and functionally analyze the role of intracellular signaling involving cyclic di-GMP in black rot disease of crucifers. Here, we describe leaf clipping and spraying methods for testing the virulence of wild type and derived mutants of X. campestris in Chinese radish. These methods address different facets of the disease cycle, which requires the ability to survive epiphytically before entry into the plant and growth and systemic spread within the xylem.

Original languageEnglish
Title of host publicationc-di-GMP Signaling
Subtitle of host publicationMethods and Protocols
EditorsKarin Sauer
Place of PublicationNew York
Number of pages8
ISBN (Electronic)9781493972401
ISBN (Print)9781493972395
Publication statusPublished - Sept 2017

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Virulence testing
  • Phytopathogenesis
  • Xanthomonas campestris
  • Leaf clipping
  • Spray inoculation


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