Probing the structure and function of U2 snRNP with antisense oligonucleotides made of 2′-OMe RNA

Angus I. Lamond, Brian Sproat, Ursula Ryder, Jörg Hamm

    Research output: Contribution to journalArticlepeer-review

    101 Citations (Scopus)

    Abstract

    We have used oligonucleotides made of 2′-OMe RNA to analyze the role of separate domains of U2 snRNA in the splicing process. We show that antisense 2′-OMe RNA oligonucleotides bind efficiently and specifically to U2 snRNP and demonstrate that masking of two separate regions of U2 snRNA can inhibit splicing by affecting different steps in the spliceosome assembly pathway. Masking the 5′ terminus of U2 snRNA does not prevent U2 snRNP binding to pre-mRNA but blocks subsequent assembly of a functional spliceosome. By contrast, masking of U2 sequences complementary to the pre-mRNA branch site completely inhibits binding of pre-mRNA. Hybrid formation at the branch site complementary region also triggers a specific change which affects the 5′ terminus of U2 snRNA.

    Original languageEnglish
    Pages (from-to)383-390
    Number of pages8
    JournalCell
    Volume58
    Issue number2
    DOIs
    Publication statusPublished - 28 Jul 1989

    ASJC Scopus subject areas

    • General Biochemistry,Genetics and Molecular Biology

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