Processing of a 58 × 103 Mr invertase from potato tubers

L. R. Burch (Lead / Corresponding author), H. V. Davies, H. A. Ross, G. C. Machray, P. Hedley, R. Waugh

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Abstract

Processing of a 58 × 103 Mr acid invertase to a 48 × 103 Mr polypeptide has been demonstrated in potato tuber extracts using antibodies raised against a synthetic N-terminal peptide. Processing of the 58 × 103 Mr protein occurred during the preparation of extracts in acetate buffer, pH 5.2, but not in Tris-HCI buffer, pH 7.5. A modified rapid extraction procedure suggested that the processing event also occurred in planta. Processing apparently increased the activity of acid invertase after foaming, a technique previously reported to dissociate the potato tuber invertase from an endogenous inhibitor. Western blot analysis revealed that the relative abundance of the 48 × 103 Mr polypeptide was correlated with acid invertase activity.

Original languageEnglish
Pages (from-to)579-582
Number of pages4
JournalPhytochemistry
Volume35
Issue number3
DOIs
Publication statusPublished - 1 Jan 1994

Keywords

  • invertase
  • potato tuber
  • processing.
  • Solanaceae
  • Solanum tuberosum

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