Production of cytochrome P450 reductase yeast-rat hybrid proteins in Saccharomyces cerevisiae

H. Frances J. Bligh, C. Roland Wolf, Gillian Smith, Jean D. Beggs

    Research output: Contribution to journalArticlepeer-review

    15 Citations (Scopus)

    Abstract

    We present a novel strategy for increasing the level of functional mammalian cytochrome P450 (Cyt.P450) and NADPH:cytochrome P450 reductase enzymes produced in yeast. A cDNA encoding the rat P450 reductase was modified by the addition of a sequence coding for the N-terminal region of P450 reductase from Saccharomyces cerevisiae. The addition of this hydrophobic tail greatly increased the apparent stability of the reductase protein produced in S. cerevisiae, as compared to the unmodified rat P450 reductase. When the rat hybrid reductase was produced simultaneously with one of two mammalian Cyt.P450s, the rat CYP2B1 or the human CYP2A6, there was a significant increase in the specific activity of each of the Cyt.P450s. The optimization of this approach and its extrapolation to other organisms should lead to a marked improvement in our ability to study and exploit the P450 system.

    Original languageEnglish
    Pages (from-to)33-9
    Number of pages7
    JournalGene
    Volume110
    Issue number1
    DOIs
    Publication statusPublished - 2 Jan 1992

    Keywords

    • Recombinant DNA
    • Hydrophobic region
    • Gene fusions
    • Mutagen metabolism

    Fingerprint

    Dive into the research topics of 'Production of cytochrome P450 reductase yeast-rat hybrid proteins in <em>Saccharomyces cerevisiae</em>'. Together they form a unique fingerprint.

    Cite this