Proinsulin is stable at room temperature for 24 hours in EDTA: A clinical laboratory analysis (adAPT 3)

Jane Davidson, Timothy J. McDonald (Lead / Corresponding author), Calum Sutherland, Mohammod Mostazir, Lidy VanAalten, Terence J. Wilkin

Research output: Contribution to journalArticle

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Abstract

Aims: Reference laboratories advise immediate separation and freezing of samples for the assay of proinsulin, which limit its practicability for smaller centres. Following the demonstration that insulin and C-peptide are stable in EDTA at room temperature for at least 24hours, we undertook simple stability studies to establish whether the same might apply to proinsulin.

Methods: Venous blood samples were drawn from six adult women, some fasting, some not, aliquoted and assayed immediately and after storage at either 4°C or ambient temperature for periods from 2h to 24h.

Results: There was no significant variation or difference with storage time or storage condition in either individual or group analysis.

Conclusion: Proinsulin appears to be stable at room temperature in EDTA for at least 24h. Immediate separation and storage on ice of samples for proinsulin assay is not necessary, which will simplify sample transport, particularly for multicentre trials.

Original languageEnglish
Article numbere0171716
Pages (from-to)1-6
Number of pages6
JournalPLoS ONE
Volume12
Issue number4
DOIs
Publication statusPublished - 20 Apr 2017

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proinsulin
Clinical laboratories
Proinsulin
Edetic Acid
ambient temperature
Temperature
Assays
sampling
C-Peptide
Ice
assays
storage conditions
Freezing
Multicenter Studies
fasting
Fasting
storage time
freezing
Blood
ice

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Davidson, Jane ; McDonald, Timothy J. ; Sutherland, Calum ; Mostazir, Mohammod ; VanAalten, Lidy ; Wilkin, Terence J. / Proinsulin is stable at room temperature for 24 hours in EDTA : A clinical laboratory analysis (adAPT 3). In: PLoS ONE. 2017 ; Vol. 12, No. 4. pp. 1-6.
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abstract = "Aims: Reference laboratories advise immediate separation and freezing of samples for the assay of proinsulin, which limit its practicability for smaller centres. Following the demonstration that insulin and C-peptide are stable in EDTA at room temperature for at least 24hours, we undertook simple stability studies to establish whether the same might apply to proinsulin.Methods: Venous blood samples were drawn from six adult women, some fasting, some not, aliquoted and assayed immediately and after storage at either 4°C or ambient temperature for periods from 2h to 24h.Results: There was no significant variation or difference with storage time or storage condition in either individual or group analysis.Conclusion: Proinsulin appears to be stable at room temperature in EDTA for at least 24h. Immediate separation and storage on ice of samples for proinsulin assay is not necessary, which will simplify sample transport, particularly for multicentre trials.",
author = "Jane Davidson and McDonald, {Timothy J.} and Calum Sutherland and Mohammod Mostazir and Lidy VanAalten and Wilkin, {Terence J.}",
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Proinsulin is stable at room temperature for 24 hours in EDTA : A clinical laboratory analysis (adAPT 3). / Davidson, Jane; McDonald, Timothy J. (Lead / Corresponding author); Sutherland, Calum; Mostazir, Mohammod; VanAalten, Lidy; Wilkin, Terence J.

In: PLoS ONE, Vol. 12, No. 4, e0171716, 20.04.2017, p. 1-6.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Proinsulin is stable at room temperature for 24 hours in EDTA

T2 - A clinical laboratory analysis (adAPT 3)

AU - Davidson, Jane

AU - McDonald, Timothy J.

AU - Sutherland, Calum

AU - Mostazir, Mohammod

AU - VanAalten, Lidy

AU - Wilkin, Terence J.

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N2 - Aims: Reference laboratories advise immediate separation and freezing of samples for the assay of proinsulin, which limit its practicability for smaller centres. Following the demonstration that insulin and C-peptide are stable in EDTA at room temperature for at least 24hours, we undertook simple stability studies to establish whether the same might apply to proinsulin.Methods: Venous blood samples were drawn from six adult women, some fasting, some not, aliquoted and assayed immediately and after storage at either 4°C or ambient temperature for periods from 2h to 24h.Results: There was no significant variation or difference with storage time or storage condition in either individual or group analysis.Conclusion: Proinsulin appears to be stable at room temperature in EDTA for at least 24h. Immediate separation and storage on ice of samples for proinsulin assay is not necessary, which will simplify sample transport, particularly for multicentre trials.

AB - Aims: Reference laboratories advise immediate separation and freezing of samples for the assay of proinsulin, which limit its practicability for smaller centres. Following the demonstration that insulin and C-peptide are stable in EDTA at room temperature for at least 24hours, we undertook simple stability studies to establish whether the same might apply to proinsulin.Methods: Venous blood samples were drawn from six adult women, some fasting, some not, aliquoted and assayed immediately and after storage at either 4°C or ambient temperature for periods from 2h to 24h.Results: There was no significant variation or difference with storage time or storage condition in either individual or group analysis.Conclusion: Proinsulin appears to be stable at room temperature in EDTA for at least 24h. Immediate separation and storage on ice of samples for proinsulin assay is not necessary, which will simplify sample transport, particularly for multicentre trials.

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