Proinsulin is stable at room temperature for 24 hours in EDTA: A clinical laboratory analysis (adAPT 3)

Jane Davidson; Timothy J. McDonald; Calum Sutherland; Mohammod Mostazir; Lidy VanAalten; Terence J. Wilkin

doi:10.1371/journal.pone.0171716

Proinsulin is stable at room temperature for 24 hours in EDTA: A clinical laboratory analysis (adAPT 3)

Jane Davidson, Timothy J. McDonald (Lead / Corresponding author), Calum Sutherland, Mohammod Mostazir, Lidy VanAalten, Terence J. Wilkin

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Abstract

Aims: Reference laboratories advise immediate separation and freezing of samples for the assay of proinsulin, which limit its practicability for smaller centres. Following the demonstration that insulin and C-peptide are stable in EDTA at room temperature for at least 24hours, we undertook simple stability studies to establish whether the same might apply to proinsulin.

Methods: Venous blood samples were drawn from six adult women, some fasting, some not, aliquoted and assayed immediately and after storage at either 4°C or ambient temperature for periods from 2h to 24h.

Results: There was no significant variation or difference with storage time or storage condition in either individual or group analysis.

Conclusion: Proinsulin appears to be stable at room temperature in EDTA for at least 24h. Immediate separation and storage on ice of samples for proinsulin assay is not necessary, which will simplify sample transport, particularly for multicentre trials.

Original language	English
Article number	e0171716
Pages (from-to)	1-6
Number of pages	6
Journal	PLoS ONE
Volume	12
Issue number	4
DOIs	https://doi.org/10.1371/journal.pone.0171716
Publication status	Published - 20 Apr 2017

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10.1371/journal.pone.0171716Licence: CC BY

Final Published Version
© 2017 Davidson et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Final published version, 720 KBLicence: CC BY

Preliminary Evaluation of the Therapeutic Potential of Lithium in Mild Cognitive Impairment (Joint with Oxford University)
Duthie, A. (Investigator) & Sutherland, C. (Investigator)
1/09/15 → 30/06/17
Project: Research

Sutherland, Calum
- Diabetes Endocrinology and Reproductive Biology - Professor (Teaching and Research) of Molecular and Cellular Diabetes
Person: Academic

Cite this

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title = "Proinsulin is stable at room temperature for 24 hours in EDTA: A clinical laboratory analysis (adAPT 3)",

abstract = "Aims: Reference laboratories advise immediate separation and freezing of samples for the assay of proinsulin, which limit its practicability for smaller centres. Following the demonstration that insulin and C-peptide are stable in EDTA at room temperature for at least 24hours, we undertook simple stability studies to establish whether the same might apply to proinsulin.Methods: Venous blood samples were drawn from six adult women, some fasting, some not, aliquoted and assayed immediately and after storage at either 4°C or ambient temperature for periods from 2h to 24h.Results: There was no significant variation or difference with storage time or storage condition in either individual or group analysis.Conclusion: Proinsulin appears to be stable at room temperature in EDTA for at least 24h. Immediate separation and storage on ice of samples for proinsulin assay is not necessary, which will simplify sample transport, particularly for multicentre trials.",

author = "Jane Davidson and McDonald, {Timothy J.} and Calum Sutherland and Mohammod Mostazir and Lidy VanAalten and Wilkin, {Terence J.}",

note = "This work was supported by Juvenile Diabetes Research Foundation grant #1-SRA-2014-54-M-A, https://jdrf.org.uk/.",

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T1 - Proinsulin is stable at room temperature for 24 hours in EDTA

T2 - A clinical laboratory analysis (adAPT 3)

AU - Davidson, Jane

AU - McDonald, Timothy J.

AU - Sutherland, Calum

AU - Mostazir, Mohammod

AU - VanAalten, Lidy

AU - Wilkin, Terence J.

N1 - This work was supported by Juvenile Diabetes Research Foundation grant #1-SRA-2014-54-M-A, https://jdrf.org.uk/.

PY - 2017/4/20

Y1 - 2017/4/20

N2 - Aims: Reference laboratories advise immediate separation and freezing of samples for the assay of proinsulin, which limit its practicability for smaller centres. Following the demonstration that insulin and C-peptide are stable in EDTA at room temperature for at least 24hours, we undertook simple stability studies to establish whether the same might apply to proinsulin.Methods: Venous blood samples were drawn from six adult women, some fasting, some not, aliquoted and assayed immediately and after storage at either 4°C or ambient temperature for periods from 2h to 24h.Results: There was no significant variation or difference with storage time or storage condition in either individual or group analysis.Conclusion: Proinsulin appears to be stable at room temperature in EDTA for at least 24h. Immediate separation and storage on ice of samples for proinsulin assay is not necessary, which will simplify sample transport, particularly for multicentre trials.

AB - Aims: Reference laboratories advise immediate separation and freezing of samples for the assay of proinsulin, which limit its practicability for smaller centres. Following the demonstration that insulin and C-peptide are stable in EDTA at room temperature for at least 24hours, we undertook simple stability studies to establish whether the same might apply to proinsulin.Methods: Venous blood samples were drawn from six adult women, some fasting, some not, aliquoted and assayed immediately and after storage at either 4°C or ambient temperature for periods from 2h to 24h.Results: There was no significant variation or difference with storage time or storage condition in either individual or group analysis.Conclusion: Proinsulin appears to be stable at room temperature in EDTA for at least 24h. Immediate separation and storage on ice of samples for proinsulin assay is not necessary, which will simplify sample transport, particularly for multicentre trials.

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Proinsulin is stable at room temperature for 24 hours in EDTA: A clinical laboratory analysis (adAPT 3)

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Preliminary Evaluation of the Therapeutic Potential of Lithium in Mild Cognitive Impairment (Joint with Oxford University)

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